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MYDGF/C19orf10 Monoclonal antibody

MYDGF/C19orf10 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1H8F4

Cat no : 68131-1-Ig

Print datasheet

Synonyms

MYDGF, C19orf10, UPF0556 protein C19orf10, SF20, Myeloid-derived growth factor

验证数据展示

  • WB analysis using 68131-1-Ig

    WB analysis using 68131-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68131-1-Ig (MYDGF/C19orf10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human liver cancer using 68131-1-Ig

    IHC staining of human liver cancer using 68131-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68131-1-Ig (MYDGF/C19orf10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYDGF antibody included
  • IHC staining of human liver cancer using 68131-1-Ig

    IHC staining of human liver cancer using 68131-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68131-1-Ig (MYDGF/C19orf10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYDGF antibody included
  • IF Staining of HepG2 using 68131-1-Ig

    IF Staining of HepG2 using 68131-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MYDGF/C19orf10 antibody (68131-1-Ig, Clone: 1H8F4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • FC experiment of HepG2 using 68131-1-Ig

    FC experiment of HepG2 using 68131-1-Ig

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human MYDGF/C19orf10 (68131-1-Ig, Clone:1H8F4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inA549 cells, LNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MYDGF Ready-To-Use IHC Kit. MYDGF primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68131-1-Ig targets MYDGF/C19orf10 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MYDGF/C19orf10 fusion protein Ag13438 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name chromosome 19 open reading frame 10
Synonyms MYDGF, C19orf10, UPF0556 protein C19orf10, SF20, Myeloid-derived growth factor
Calculated Molecular Weight 173 aa, 19 kDa
Observed Molecular Weight15-19 kDa
GenBank Accession NumberBC010129
Gene Symbol MYDGF/C19orf10
Gene ID (NCBI) 56005
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ969H8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Myeloid-derived growth factor (MYDGF), also named as chromosome 19 open reading frame 10 (C19orf10), is an FLS-derived protein that is secreted into the synovial fluid. MYDGF as a paracrine-acting protein secreted from monocytes/macrophages that improves tissue repair and heart function after myocardial infarction. MYDGF has been reported to regulates neutrophil motility in interstitial tissue damage(PMID: 34047769).

实验方案

Product Specific Protocols
WB protocol for MYDGF/C19orf10 antibody 68131-1-IgDownload protocol
IHC protocol for MYDGF/C19orf10 antibody 68131-1-IgDownload protocol
IF protocol for MYDGF/C19orf10 antibody 68131-1-IgDownload protocol
FC protocol for MYDGF/C19orf10 antibody 68131-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols