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C1D Polyclonal antibody

C1D Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10711-1-AP

Print datasheet

Synonyms

C1D, hC1D, LRP1, SUN CoR, SUNCOR

验证数据展示

  • IP experiment of DU 145 using 10711-1-AP

    IP experiment of DU 145 using 10711-1-AP

    IP result of anti-C1D (IP:10711-1-AP, 4ug; Detection:10711-1-AP 1:300) with DU 145 cells lysate 1800 ug.

  • WB analysis of DU 145 using 10711-1-AP

    WB analysis of DU 145 using 10711-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 10711-1-AP (C1D Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 10711-1-AP

    IHC staining of human prostate cancer using 10711-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10711-1-AP (C1D Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with C1D antibody included
  • IHC staining of human prostate cancer using 10711-1-AP

    IHC staining of human prostate cancer using 10711-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10711-1-AP (C1D Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with C1D antibody included

经过测试的应用

Positive WB detected inDU 145 cells
Positive IP detected inDU 145 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy C1D Ready-To-Use IHC Kit. C1D primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

10711-1-AP targets C1D in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivitymouse
Immunogen C1D fusion protein Ag1121 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name C1D nuclear receptor co-repressor
Synonyms C1D, hC1D, LRP1, SUN CoR, SUNCOR
Calculated Molecular Weight 16 kDa
GenBank Accession NumberBC005235
Gene Symbol C1D
Gene ID (NCBI) 10438
RRIDAB_2067014
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13901
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C1D antibody 10711-1-APDownload protocol
IHC protocol for C1D antibody 10711-1-APDownload protocol
IP protocol for C1D antibody 10711-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Redox Biol

Multi-omics profiling reveals Chitinase-3-like protein 1 as a key mediator in the crosstalk between sarcopenia and liver cancer

Authors - Di Lu
View Article