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C1QBP Monoclonal antibody

C1QBP Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1F9B1

Cat no : 68084-1-Ig

Print datasheet

Synonyms

gC1Q R, GC1QBP, GC1q R protein, Complement component 1 Q subcomponent-binding protein, mitochondrial, ASF/SF2-associated protein p32

验证数据展示

  • WB analysis using 68084-1-Ig

    WB analysis using 68084-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68084-1-Ig (C1QBP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • IF Staining of HeLa using 68084-1-Ig

    IF Staining of HeLa using 68084-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using C1QBP antibody (68084-1-Ig, Clone: 1F9B1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IHC staining of human tonsillitis using 68084-1-Ig

    IHC staining of human tonsillitis using 68084-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68084-1-Ig (C1QBP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with C1QBP antibody included
  • IHC staining of human tonsillitis using 68084-1-Ig

    IHC staining of human tonsillitis using 68084-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68084-1-Ig (C1QBP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with C1QBP antibody included
  • FC experiment of HeLa using 68084-1-Ig

    FC experiment of HeLa using 68084-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human C1QBP (68084-1-Ig, Clone:1F9B1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inLNCaP cells, mouse brain tissue, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman tonsillitis tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy C1QBP Ready-To-Use IHC Kit. C1QBP primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68084-1-Ig targets C1QBP in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse
Immunogen C1QBP fusion protein Ag19773 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name complement component 1, q subcomponent binding protein
Synonyms gC1Q R, GC1QBP, GC1q R protein, Complement component 1 Q subcomponent-binding protein, mitochondrial, ASF/SF2-associated protein p32
Calculated Molecular Weight 282 aa, 31 kDa
Observed Molecular Weight32 kDa
GenBank Accession NumberBC013731
Gene Symbol C1QBP
Gene ID (NCBI) 708
RRIDAB_2918821
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ07021
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C1QBP, also named as gC1q receptor (gC1qR), p32, p33, and hyaluronan-binding protein 1 (HABP1), is a protein initially copurified with splicing factor SF2 (PMID: 1830244). The protein is synthesized as a pro-protein of 282 amino acids (aa) that is post-translationally processed by removal of the initial 73 aa to a mature protein of 209 aa (PMID: 8262387). C1QBP is an evolutionary conserved and ubiquitously expressed multifunctional protein and has been reported to be a predominantly mitochondrial matrix protein involved in inflammation and infection processes, mitochondrial ribosome biogenesis, regulation of apoptosis and nuclear transcription, and pre-mRNA splicing (PMID: 28942965).

实验方案

Product Specific Protocols
WB protocol for C1QBP antibody 68084-1-IgDownload protocol
IHC protocol for C1QBP antibody 68084-1-IgDownload protocol
IF protocol for C1QBP antibody 68084-1-IgDownload protocol
FC protocol for C1QBP antibody 68084-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols