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C1orf83 Recombinant antibody

C1orf83 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240678F1

Cat no : 83626-5-RR

Print datasheet

Synonyms

Transcription elongation factor A N-terminal and central domain-containing protein 2, TCEANC2, RP4 758J24.3, FLJ39169, FLJ32112

验证数据展示

  • WB analysis using 83626-5-RR

    WB analysis using 83626-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83626-5-RR (C1orf83 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 83626-5-RR

    IF Staining of U2OS using 83626-5-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using C1orf83 antibody (83626-5-RR, Clone: 240678F1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83626-5-RR

    Affinity and Kinetic Characterization of 83626-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83626-5-RR against Human C1orf83 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inU2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83626-5-RR is C1orf83 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen C1orf83 fusion protein Ag15040 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chromosome 1 open reading frame 83
Synonyms Transcription elongation factor A N-terminal and central domain-containing protein 2, TCEANC2, RP4 758J24.3, FLJ39169, FLJ32112
Calculated Molecular Weight 208 aa, 23 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC035374
Gene Symbol C1orf83
Gene ID (NCBI) 127428
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ96MN5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Chromosome 1 open reading frame 83 (C1orf83) is also called TCEANC2, a little-studied transcription elongation factor. The rs10788972 variant of the TCEANC2 gene in the PARK10 region is closely associated with sporadic PD in the American population, while both rs10788972 and rs12046178 variants are not associated with sporadic PD in the Chinese Han population (PMID: 25663231; PMID: 26432391).

实验方案

Product Specific Protocols
WB protocol for C1orf83 antibody 83626-5-RRDownload protocol
IF protocol for C1orf83 antibody 83626-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols