TMEM230 Polyclonal antibody

TMEM230 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 21466-1-AP

Print datasheet

Synonyms

C20orf30, HSPC274, TMEM230, UNQ2432/PRO4992



经过测试的应用

Positive WB detected inHepG2 cells, COLO 320 cells, HEK-293 cells, HeLa cells
Positive IHC detected inhuman brain tissue, human colon cancer tissue, human liver cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21466-1-AP is TMEM230 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF, IHC
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen TMEM230 fusion protein Ag15080 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 20 open reading frame 30
Synonyms C20orf30, HSPC274, TMEM230, UNQ2432/PRO4992
Calculated Molecular Weight 183 aa, 20 kDa
Observed Molecular Weight 13 kDa
GenBank Accession NumberBC011990
Gene Symbol TMEM230
Gene ID (NCBI) 29058
RRIDAB_10791969
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96A57
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The function of TMEM230/C20orf30 has not been widely studied, and is yet to be fully elucidated. TMEM230 is primarily a transmembrane protein of synaptic vesicles in neurons. Disease-linked TMEM230 mutants impair synaptic vesicle trafficking leading to Parkinson's disease. Two isoforms were produced by alternative splicing with predicted MW of 13 and 20 kDa. 13 kDa isoform accounts for >95% of the total protein isoforms(27270108).

实验方案

Product Specific Protocols
WB protocol for TMEM230 antibody 21466-1-APDownload protocol
IHC protocol for TMEM230 antibody 21466-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Genet

Identification of TMEM230 mutations in familial Parkinson's disease.

Authors - Han-Xiang Deng
humanWB

Nat Cell Biol

Molecular identification of a BAR domain-containing coat complex for endosomal recycling of transmembrane proteins.

Authors - Boris Simonetti
humanWB,IHC

J Alzheimers Dis

TMEM230 Accumulation in Granulovacuolar Degeneration Bodies and Dystrophic Neurites of Alzheimer's Disease

Authors - Sandra L Siedlak
mouseWB,IF

Exp Anim

TMEM230 modulates seizure in epilepsy: evidence from temporal lobe epilepsy patients and mouse models

Authors - Haiqing Zhang
humanWB

Int J Mol Sci

Transmembrane Protein TMEM230, Regulator of Glial Cell Vascular Mimicry and Endothelial Cell Angiogenesis in High-Grade Heterogeneous Infiltrating Gliomas and Glioblastoma

Authors - Cinzia Cocola