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C21orf66 Polyclonal antibody

C21orf66 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21357-1-AP

Print datasheet

Synonyms

PAX3- and PAX7-binding protein 1, GC-rich sequence DNA-binding factor 1, GCFC1, GCFC, fSAP105

验证数据展示

  • WB analysis of human brain using 21357-1-AP

    WB analysis of human brain using 21357-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 21357-1-AP (C21orf66 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 21357-1-AP

    IF Staining of HeLa using 21357-1-AP

    Immunofluorescent analysis of Hela cells, using C21orf66 antibody 21357-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IHC staining of mouse liver using 21357-1-AP

    IHC staining of mouse liver using 21357-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 21357-1-AP (C21orf66 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 21357-1-AP

    IHC staining of human pancreas cancer using 21357-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 21357-1-AP (C21orf66 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human renal cell carcinoma using 21357-1-AP

    IHC staining of human renal cell carcinoma using 21357-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 21357-1-AP (C21orf66 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human renal cell carcinoma using 21357-1-AP

    IHC staining of human renal cell carcinoma using 21357-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 21357-1-AP (C21orf66 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman renal cell carcinoma tissue, human pancreas cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

21357-1-AP targets C21orf66 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen C21orf66 fusion protein Ag15789 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 21 open reading frame 66
Synonyms PAX3- and PAX7-binding protein 1, GC-rich sequence DNA-binding factor 1, GCFC1, GCFC, fSAP105
Calculated Molecular Weight 917 aa, 105 kDa
Observed Molecular Weight 93 kDa
GenBank Accession NumberBC030539
Gene Symbol C21orf66
Gene ID (NCBI) 94104
RRIDAB_10733239
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y5B6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C21orf66 antibody 21357-1-APDownload protocol
IHC protocol for C21orf66 antibody 21357-1-APDownload protocol
IF protocol for C21orf66 antibody 21357-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Front Immunol

Paxbp1 is indispensable for the survival of CD4 and CD8 double-positive thymocytes

Authors - Wenting Li
View Article
mouseWB

Immunology

Paxbp1 is indispensable for the maintenance of peripheral CD4 T cell homeostasis

Authors - Wenting Li
View Article