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验证数据展示

  • WB analysis using 66157-1-Ig

    WB analysis using 66157-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66157-1-Ig (C3/C3b/C3c antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 66157-1-Ig

    IHC staining of human liver using 66157-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66157-1-Ig (C3/C3b/C3c antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with C3 antibody included
  • IHC staining of human liver using 66157-1-Ig

    IHC staining of human liver using 66157-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66157-1-Ig (C3/C3b/C3c antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with C3 antibody included
  • IF Staining of HepG2 using 66157-1-Ig

    IF Staining of HepG2 using 66157-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66157-1-Ig (C3/C3b/C3c antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

C3/C3b/C3c Monoclonal antibody

C3/C3b/C3c Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66157-1-Ig

产品说明书

CloneNo. 2A6B3
引用文献(3)

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

C3, C3/C3a, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, ASP, ARMD9

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHUVEC cells, human blood, human plasma, HepG2 cells, J774A.1 cells, RAW 264.7 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy C3 Ready-To-Use IHC Kit. C3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66157-1-Ig targets C3/C3b/C3c in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF, ELISA
经测试反应性 human, mouse
文献引用反应性mouse, rat
免疫原 C3/C3b/C3c fusion protein Ag15955 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 complement component 3
别名 C3, C3/C3a, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, ASP, ARMD9
计算分子量 1663 aa, 187 kDa
观测分子量 115 kDa
GenBank蛋白编号BC150179
基因名称 C3
Gene ID (NCBI) 718
RRIDAB_2881553
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP01024
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The complement system is an important effector that bridges the innate and adaptive immune systems (PMID: 20010915). The third component of complement, C3, plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways (PMID: 11414361). Human C3, composed of α and β chains (115 and 75 kDa, respectively), is cleaved into C3a and C3b by C3 convertase. C3b is further cleaved into iC3b, C3c, C3dg and C3f. This antibody raised against 1314-1663 aa of human C3 protein recognizes the C3 alpha chain, C3b alpha' chain, and C3c alpha' chain fragment 2.

实验方案

Product Specific Protocols
WB protocol for C3/C3b/C3c antibody 66157-1-IgDownload protocol
IHC protocol for C3/C3b/C3c antibody 66157-1-IgDownload protocol
IF protocol for C3/C3b/C3c antibody 66157-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB,IF

J Neuroinflammation

Apelin alleviated neuroinflammation and promoted endogenous neural stem cell proliferation and differentiation after spinal cord injury in rats.

Authors - Qing Liu
View Article
mouseWB,ELISA

Virulence

HylS', a fragment of truncated hyaluronidase of Streptococcus suis, contributes to immune evasion by interaction with host complement factor C3b

Authors - Jiajia Xu
View Article
ratWB

Neurochem Int

Quantitative iTRAQ-based proteomic analysis of piperine protected cerebral ischemia/reperfusion injury in rat brain.

Authors - Yichun Zou
View Article