• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

C3/C3b/C3c Monoclonal antibody, PBS Only

C3/C3b/C3c Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2A6B3

Cat no : 66157-1-PBS

Print datasheet

Synonyms

C3, C3/C3a, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, ASP, ARMD9

验证数据展示

  • WB analysis using 66157-1-Ig (same clone as 66157-1-PBS)

    WB analysis using 66157-1-Ig (same clone as 66157-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66157-1-Ig (C3/C3b/C3c antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66157-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66157-1-Ig (same clone as 66157-1-PBS)

    IHC staining of human liver using 66157-1-Ig (same clone as 66157-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66157-1-Ig (C3/C3b/C3c antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66157-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with C3 antibody included
  • IHC staining of human liver using 66157-1-Ig (same clone as 66157-1-PBS)

    IHC staining of human liver using 66157-1-Ig (same clone as 66157-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66157-1-Ig (C3/C3b/C3c antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66157-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with C3 antibody included
  • IF Staining of HepG2 using 66157-1-Ig (same clone as 66157-1-PBS)

    IF Staining of HepG2 using 66157-1-Ig (same clone as 66157-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66157-1-Ig (C3/C3b/C3c antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66157-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy C3 Ready-To-Use IHC Kit. C3 primary antibody included.

产品信息

66157-1-PBS targets C3/C3b/C3c in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen C3/C3b/C3c fusion protein Ag15955 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name complement component 3
Synonyms C3, C3/C3a, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, ASP, ARMD9
Calculated Molecular Weight 1663 aa, 187 kDa
Observed Molecular Weight 115 kDa
GenBank Accession NumberBC150179
Gene Symbol C3
Gene ID (NCBI) 718
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP01024
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The complement system is an important effector that bridges the innate and adaptive immune systems (PMID: 20010915). The third component of complement, C3, plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways (PMID: 11414361). Human C3, composed of α and β chains (115 and 75 kDa, respectively), is cleaved into C3a and C3b by C3 convertase. C3b is further cleaved into iC3b, C3c, C3dg and C3f. This antibody raised against 1314-1663 aa of human C3 protein recognizes the C3 alpha chain, C3b alpha' chain, and C3c alpha' chain fragment 2.