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C3/C3b/C3c Monoclonal antibody, PBS Only

C3/C3b/C3c Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2A6B3

Cat no : 66157-1-PBS

Print datasheet

Synonyms

C3, C3/C3a, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, ASP, ARMD9

验证数据展示

  • WB analysis using 66157-1-Ig (same clone as 66157-1-PBS)

    WB analysis using 66157-1-Ig (same clone as 66157-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66157-1-Ig (C3/C3b/C3c antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66157-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66157-1-Ig (same clone as 66157-1-PBS)

    IHC staining of human liver using 66157-1-Ig (same clone as 66157-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66157-1-Ig (C3/C3b/C3c antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66157-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with C3 antibody included
  • IHC staining of human liver using 66157-1-Ig (same clone as 66157-1-PBS)

    IHC staining of human liver using 66157-1-Ig (same clone as 66157-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66157-1-Ig (C3/C3b/C3c antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66157-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with C3 antibody included
  • IF Staining of HepG2 using 66157-1-Ig (same clone as 66157-1-PBS)

    IF Staining of HepG2 using 66157-1-Ig (same clone as 66157-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66157-1-Ig (C3/C3b/C3c antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66157-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy C3 Ready-To-Use IHC Kit. C3 primary antibody included.

产品信息

66157-1-PBS targets C3/C3b/C3c in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen C3/C3b/C3c fusion protein Ag15955 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name complement component 3
Synonyms C3, C3/C3a, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, ASP, ARMD9
Calculated Molecular Weight 1663 aa, 187 kDa
Observed Molecular Weight 115 kDa
GenBank Accession NumberBC150179
Gene Symbol C3
Gene ID (NCBI) 718
RRIDAB_2881553
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP01024
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The complement system is an important effector that bridges the innate and adaptive immune systems (PMID: 20010915). The third component of complement, C3, plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways (PMID: 11414361). Human C3, composed of α and β chains (115 and 75 kDa, respectively), is cleaved into C3a and C3b by C3 convertase. C3b is further cleaved into iC3b, C3c, C3dg and C3f. This antibody raised against 1314-1663 aa of human C3 protein recognizes the C3 alpha chain, C3b alpha' chain, and C3c alpha' chain fragment 2.