• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

C4 Gamma Chain Monoclonal antibody, PBS Only

C4 Gamma Chain Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G6A7

Cat no : 66242-1-PBS

Print datasheet

Synonyms

C4A, C4 (gamma chain), C4A2, C4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2

验证数据展示

  • WB analysis of human plasma using 66242-1-Ig (same clone as 66242-1-PBS)

    WB analysis of human plasma using 66242-1-Ig (same clone as 66242-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66242-1-Ig (C4 gamma chain antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66242-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66242-1-Ig (same clone as 66242-1-PBS)

    IHC staining of human liver using 66242-1-Ig (same clone as 66242-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66242-1-Ig (C4 gamma chain antibody) at dilution of 1:800 (under 10x lens). This data was developed using the same antibody clone with 66242-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66242-1-Ig (same clone as 66242-1-PBS)

    IHC staining of human liver using 66242-1-Ig (same clone as 66242-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66242-1-Ig (C4 gamma chain antibody) at dilution of 1:800 (under 40x lens). This data was developed using the same antibody clone with 66242-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver using 66242-1-Ig (same clone as 66242-1-PBS)

    IF Staining of human liver using 66242-1-Ig (same clone as 66242-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using C4 Gamma Chain antibody (66242-1-Ig, Clone: 2G6A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66242-1-PBS in a different storage buffer formulation.

产品信息

66242-1-PBS targets C4 Gamma Chain in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen C4 Gamma Chain fusion protein Ag10449 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name complement component 4A (Rodgers blood group)
Synonyms C4A, C4 (gamma chain), C4A2, C4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2
Calculated Molecular Weight 1744 aa, 193 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC012372
Gene Symbol C4 Gamma Chain
Gene ID (NCBI) 720
RRIDAB_2881631
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP0C0L4
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Complement C4, a component of the classical complement pathway, has an important role in innate immune function. C4 protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains (molecular wights 95, 78, and 31 kDa) prior to secretion. The alpha chain is cleaved to release C4 anaphylatoxin, an antimicrobial peptide and a mediator of local inflammation. This antibody raised against 1543-1744aa of human C4-A protein recognizes C4 gamma chain.