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验证数据展示

  • WB analysis of human plasma using 66242-1-Ig

    WB analysis of human plasma using 66242-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66242-1-Ig (C4 gamma chain antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 66242-1-Ig

    IHC staining of human liver using 66242-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66242-1-Ig (C4 gamma chain antibody) at dilution of 1:800 (under 10x lens).

  • IHC staining of human liver using 66242-1-Ig

    IHC staining of human liver using 66242-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66242-1-Ig (C4 gamma chain antibody) at dilution of 1:800 (under 40x lens).

  • IF Staining of human liver using 66242-1-Ig

    IF Staining of human liver using 66242-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using C4 Gamma Chain antibody (66242-1-Ig, Clone: 2G6A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

C4 Gamma Chain Monoclonal antibody

C4 Gamma Chain Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66242-1-Ig

Print datasheet

CloneNo. 2G6A7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Synonyms

C4A, C4 (gamma chain), C4A2, C4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inhuman plasma tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66242-1-Ig targets C4 Gamma Chain in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity human, mouse
Immunogen C4 Gamma Chain fusion protein Ag10449 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name complement component 4A (Rodgers blood group)
Synonyms C4A, C4 (gamma chain), C4A2, C4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2
Calculated Molecular Weight 1744 aa, 193 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC012372
Gene Symbol C4 Gamma Chain
Gene ID (NCBI) 720
RRIDAB_2881631
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP0C0L4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Complement C4, a component of the classical complement pathway, has an important role in innate immune function. C4 protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains (molecular wights 95, 78, and 31 kDa) prior to secretion. The alpha chain is cleaved to release C4 anaphylatoxin, an antimicrobial peptide and a mediator of local inflammation. This antibody raised against 1543-1744aa of human C4-A protein recognizes C4 gamma chain.

实验方案

Product Specific Protocols
WB protocol for C4 Gamma Chain antibody 66242-1-IgDownload protocol
IHC protocol for C4 Gamma Chain antibody 66242-1-IgDownload protocol
IF protocol for C4 Gamma Chain antibody 66242-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols