验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse liver tissue |
For other applications, we recommend the unconjugated version of this antibody, 66242-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66242 targets C4 Gamma Chain in IF-P applications and shows reactivity with human samples.
| 经测试应用 | IF-P Application Description |
| 经测试反应性 | human |
| 免疫原 | C4 Gamma Chain fusion protein Ag10449 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | complement component 4A (Rodgers blood group) |
| 别名 | C4A, C4 (gamma chain), C4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2, Acidic complement C4 |
| 计算分子量 | 1744 aa, 193 kDa |
| 观测分子量 | 31 kDa |
| GenBank蛋白编号 | BC012372 |
| 基因名称 | C4 Gamma Chain |
| Gene ID (NCBI) | 720 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P0C0L4 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Complement C4, a component of the classical complement pathway, has an important role in innate immune function. C4 protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains (molecular wights 95, 78, and 31 kDa) prior to secretion. The alpha chain is cleaved to release C4 anaphylatoxin, an antimicrobial peptide and a mediator of local inflammation. This antibody raised against 1543-1744aa of human C4-A protein recognizes C4 gamma chain.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 C4 Gamma Chain antibody CL488-66242 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |