• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

C5 Monoclonal antibody, PBS Only

C5 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2G8C9

Cat no : 66634-1-PBS

Print datasheet

Synonyms

C5, C5b, C5, C5a, Complement C5, C5a anaphylatoxin, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4

验证数据展示

  • WB analysis of human plasma using 66634-1-Ig (same clone as 66634-1-PBS)

    WB analysis of human plasma using 66634-1-Ig (same clone as 66634-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66634-1-Ig (C5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66634-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 66634-1-Ig (same clone as 66634-1-PBS)

    FC experiment of HepG2 using 66634-1-Ig (same clone as 66634-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human C5 (66634-1-Ig, Clone:2G8C9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66634-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66634-1-Ig (same clone as 66634-1-PBS)

    IF Staining of HepG2 using 66634-1-Ig (same clone as 66634-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using C5 antibody (66634-1-Ig, Clone: 2G8C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66634-1-PBS in a different storage buffer formulation.

产品信息

66634-1-PBS targets C5 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen C5 fusion protein Ag17882 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name complement component 5
Synonyms C5, C5b, C5, C5a, Complement C5, C5a anaphylatoxin, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
Calculated Molecular Weight 1676 aa, 188 kDa
Observed Molecular Weight 115 kDa
GenBank Accession NumberBC113740
Gene Symbol C5
Gene ID (NCBI) 727
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01031
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C