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Conjugate
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验证数据展示

  • WB analysis of human plasma using 66634-1-Ig (same clone as 66634-1-PBS)

    WB analysis of human plasma using 66634-1-Ig (same clone as 66634-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66634-1-Ig (C5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66634-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66634-1-Ig (same clone as 66634-1-PBS)

    IF Staining of HepG2 using 66634-1-Ig (same clone as 66634-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using C5 antibody (66634-1-Ig, Clone: 2G8C9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66634-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 66634-1-Ig (same clone as 66634-1-PBS)

    FC experiment of HepG2 using 66634-1-Ig (same clone as 66634-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human C5 (66634-1-Ig, Clone:2G8C9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66634-1-PBS in a different storage buffer formulation.

C5 Monoclonal antibody, PBS Only

C5 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 66634-1-PBS

产品说明书

CloneNo. 2G8C9

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

C5, C5b, C5, C5a, Complement C5, C5a anaphylatoxin, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66634-1-PBS targets C5 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 C5 fusion protein Ag17882 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 complement component 5
别名 C5, C5b, C5, C5a, Complement C5, C5a anaphylatoxin, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
计算分子量 1676 aa, 188 kDa
观测分子量 115 kDa
GenBank蛋白编号BC113740
基因名称 C5
Gene ID (NCBI) 727
RRIDAB_2881993
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP01031
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The complement system is an important effector that bridges the innate and adaptive immune systems. The fifth component of complement, C5, is part of the complement cascade and plays an important role in inflammation and cell killing. It consists of disulfide-linked alpha and beta polypeptide chains but can be cleaved into two active peptides (C5a and C5b) by C5 convertases. Mutations and defects in C5 are associated with severe recurrent infections. (PMID: 20010915; PMID: 8011297; PMID: 6554279)