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C5 Monoclonal antibody

C5 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2G8C9

Cat no : 66634-1-Ig

Print datasheet

Synonyms

C5, C5b, C5, C5a, Complement C5, C5a anaphylatoxin, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4

验证数据展示

  • WB analysis of human plasma using 66634-1-Ig

    WB analysis of human plasma using 66634-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66634-1-Ig (C5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 66634-1-Ig

    FC experiment of HepG2 using 66634-1-Ig

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human C5 (66634-1-Ig, Clone:2G8C9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using 66634-1-Ig

    IF Staining of HepG2 using 66634-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using C5 antibody (66634-1-Ig, Clone: 2G8C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inhuman plasma tissue, rabbit serum
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66634-1-Ig targets C5 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Cited ApplicationsWB, ELISA
Tested Reactivity human
Cited Reactivityhuman
Immunogen C5 fusion protein Ag17882 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name complement component 5
Synonyms C5, C5b, C5, C5a, Complement C5, C5a anaphylatoxin, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
Calculated Molecular Weight 1676 aa, 188 kDa
Observed Molecular Weight 115 kDa
GenBank Accession NumberBC113740
Gene Symbol C5
Gene ID (NCBI) 727
RRIDAB_2881993
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01031
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C5 antibody 66634-1-IgDownload protocol
IF protocol for C5 antibody 66634-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell Proteomics

Proteomic landscape of exosomes reveals the functional contributions of CD151 in triple-negative breast cancer.

Authors - Sipeng Li
View Article
humanWB

Sci Rep

Natural antibodies and CRP drive anaphylatoxin production by urate crystals.

Authors - Anne Kathrin Wessig
View Article
ELISA

Virulence

Streptococcus suis subtilisin-like serine proteases SspA-1 and SspA-2 interplay with complement C3a and C5a to facilitate bacterial immune evasion and infection

Authors - Simin Deng
View Article