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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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C5 Monoclonal antibody

C5 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2G8C9

Cat No : 66634-1-Ig

Print datasheet

Synonyms

C5, Complement C5, complement component 5, CPAMD4

验证数据展示

  • WB analysis of human plasma using 66634-1-Ig

    WB analysis of human plasma using 66634-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66634-1-Ig (C5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 66634-1-Ig

    IF Staining of HepG2 using 66634-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using C5 antibody (66634-1-Ig, Clone: 2G8C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inhuman plasma tissue
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66634-1-Ig targets C5 in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, ELISA
Tested Reactivity Human
Cited Reactivityhuman
Immunogen C5 fusion protein Ag17882 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name complement component 5
Synonyms C5, Complement C5, complement component 5, CPAMD4
Calculated Molecular Weight 1676 aa, 188 kDa
Observed Molecular Weight 115 kDa
GenBank Accession NumberBC113740
Gene Symbol C5
Gene ID (NCBI) 727
RRIDAB_2881993
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01031
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for C5 antibody 66634-1-IgDownload protocol
IF protocol for C5 antibody 66634-1-IgDownload protocol
FC protocol for C5 antibody 66634-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell Proteomics

Proteomic landscape of exosomes reveals the functional contributions of CD151 in triple-negative breast cancer.

Authors - Sipeng Li
View Article
humanWB

Sci Rep

Natural antibodies and CRP drive anaphylatoxin production by urate crystals.

Authors - Anne Kathrin Wessig
View Article
ELISA

Virulence

Streptococcus suis subtilisin-like serine proteases SspA-1 and SspA-2 interplay with complement C3a and C5a to facilitate bacterial immune evasion and infection

Authors - Simin Deng
View Article