• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CA12 Monoclonal antibody, PBS Only

CA12 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E1D8

Cat no : 68224-1-PBS

Print datasheet

Synonyms

CA XII, CA12, Carbonate dehydratase XII, Carbonic anhydrase 12, carbonic anhydrase XII, CAXII, FLJ20151, HsT18816, Tumor antigen HOM RCC 3.1.3

验证数据展示

  • WB analysis using 68224-1-Ig (same clone as 68224-1-PBS)

    WB analysis using 68224-1-Ig (same clone as 68224-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68224-1-Ig (CA12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68224-1-PBS in a different storage buffer formulation.

  • WB analysis using 68224-1-Ig (same clone as 68224-1-PBS)

    WB analysis using 68224-1-Ig (same clone as 68224-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68224-1-Ig (CA12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68224-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 68224-1-Ig (same clone as 68224-1-PBS)

    IF Staining of A549 using 68224-1-Ig (same clone as 68224-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CA12 antibody (68224-1-Ig, Clone: 3E1D8 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68224-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CA12 Ready-To-Use IHC Kit. CA12 primary antibody included.

产品信息

68224-1-PBS targets CA12 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen CA12 fusion protein Ag7235 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name carbonic anhydrase XII
Synonyms CA XII, CA12, Carbonate dehydratase XII, Carbonic anhydrase 12, carbonic anhydrase XII, CAXII, FLJ20151, HsT18816, Tumor antigen HOM RCC 3.1.3
Calculated Molecular Weight 39 kDa
Observed Molecular Weight38-45 kDa
GenBank Accession NumberBC000278
Gene Symbol CA12
Gene ID (NCBI) 771
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO43570
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CA12 belongs to the alpha-carbonic anhydrase family. It reversible hydration of carbon dioxide. Overexpression of the zinc enzyme CA12 is observed in certain human cancers. The structure reveals a prototypical CA fold; however, two CA12 domains associate to form an isologous dimer, an observation that is confirmed by studies of the enzyme in solution.