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验证数据展示

  • WB analysis using 68224-1-Ig

    WB analysis using 68224-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68224-1-Ig (CA12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68224-1-Ig

    WB analysis using 68224-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68224-1-Ig (CA12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 68224-1-Ig

    IF Staining of A549 using 68224-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CA12 antibody (68224-1-Ig, Clone: 3E1D8 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

CA12 Monoclonal antibody

CA12 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68224-1-Ig

Print datasheet

CloneNo. 3E1D8

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

CA XII, CA12, Carbonate dehydratase XII, Carbonic anhydrase 12, carbonic anhydrase XII, CAXII, FLJ20151, HsT18816, Tumor antigen HOM RCC 3.1.3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, LNCaP cells, MCF-7 cells, HeLa cells
Positive IF/ICC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy CA12 Ready-To-Use IHC Kit. CA12 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68224-1-Ig targets CA12 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen CA12 fusion protein Ag7235 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name carbonic anhydrase XII
Synonyms CA XII, CA12, Carbonate dehydratase XII, Carbonic anhydrase 12, carbonic anhydrase XII, CAXII, FLJ20151, HsT18816, Tumor antigen HOM RCC 3.1.3
Calculated Molecular Weight 39 kDa
Observed Molecular Weight38-45 kDa
GenBank Accession NumberBC000278
Gene Symbol CA12
Gene ID (NCBI) 771
RRIDAB_2935312
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO43570
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CA12 belongs to the alpha-carbonic anhydrase family. It reversible hydration of carbon dioxide. Overexpression of the zinc enzyme CA12 is observed in certain human cancers. The structure reveals a prototypical CA fold; however, two CA12 domains associate to form an isologous dimer, an observation that is confirmed by studies of the enzyme in solution.

实验方案

Product Specific Protocols
WB protocol for CA12 antibody 68224-1-IgDownload protocol
IF protocol for CA12 antibody 68224-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols