验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, LNCaP cells, MCF-7 cells, HeLa cells |
Positive IF/ICC detected in | A549 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68224-1-Ig targets CA12 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
经测试反应性 | Human |
免疫原 | CA12 fusion protein Ag7235 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | carbonic anhydrase XII |
别名 | CA XII, CA12, Carbonate dehydratase XII, Carbonic anhydrase 12, carbonic anhydrase XII, CAXII, FLJ20151, HsT18816, Tumor antigen HOM RCC 3.1.3 |
计算分子量 | 39 kDa |
观测分子量 | 38-45 kDa |
GenBank蛋白编号 | BC000278 |
基因名称 | CA12 |
Gene ID (NCBI) | 771 |
RRID | AB_2935312 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | O43570 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CA12 belongs to the alpha-carbonic anhydrase family. It reversible hydration of carbon dioxide. Overexpression of the zinc enzyme CA12 is observed in certain human cancers. The structure reveals a prototypical CA fold; however, two CA12 domains associate to form an isologous dimer, an observation that is confirmed by studies of the enzyme in solution.
实验方案
Product Specific Protocols | |
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WB protocol for CA12 antibody 68224-1-Ig | Download protocol |
IF protocol for CA12 antibody 68224-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |