CoraLite® Plus 488-conjugated CA12 Monoclonal antibody

CA12 Monoclonal Antibody for IF/ICC
Cat No. CL488-68224

产品说明书

CloneNo. 3E1D8

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

IF/ICC

CA XII, CA12, Carbonate dehydratase XII, Carbonic anhydrase 12, carbonic anhydrase XII, CAXII, FLJ20151, HsT18816, Tumor antigen HOM RCC 3.1.3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy CA12 Ready-To-Use IHC Kit. CA12 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68224-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68224 targets CA12 in IF/ICC applications and shows reactivity with Human samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human
免疫原 CA12 fusion protein Ag7235 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 carbonic anhydrase XII
别名 CA XII, CA12, Carbonate dehydratase XII, Carbonic anhydrase 12, carbonic anhydrase XII, CAXII, FLJ20151, HsT18816, Tumor antigen HOM RCC 3.1.3
计算分子量 39 kDa
观测分子量38-45 kDa
GenBank蛋白编号BC000278
基因名称 CA12
Gene ID (NCBI) 771
RRIDAB_3672979
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO43570
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CA12 belongs to the alpha-carbonic anhydrase family. It reversible hydration of carbon dioxide. Overexpression of the zinc enzyme CA12 is observed in certain human cancers. The structure reveals a prototypical CA fold; however, two CA12 domains associate to form an isologous dimer, an observation that is confirmed by studies of the enzyme in solution.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CA12 antibody CL488-68224Download protocol
Standard Protocols
Click here to view our Standard Protocols
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