验证数据展示
经过测试的应用
Positive IF/ICC detected in | A549 cells |
Planning an IHC experiment? We recommend our IHCeasy CA12 Ready-To-Use IHC Kit. CA12 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68224-1-Ig
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-68224 targets CA12 in IF/ICC applications and shows reactivity with Human samples.
经测试应用 | IF/ICC Application Description |
经测试反应性 | Human |
免疫原 | CA12 fusion protein Ag7235 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | carbonic anhydrase XII |
别名 | CA XII, CA12, Carbonate dehydratase XII, Carbonic anhydrase 12, carbonic anhydrase XII, CAXII, FLJ20151, HsT18816, Tumor antigen HOM RCC 3.1.3 |
计算分子量 | 39 kDa |
观测分子量 | 38-45 kDa |
GenBank蛋白编号 | BC000278 |
基因名称 | CA12 |
Gene ID (NCBI) | 771 |
RRID | AB_3672979 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | O43570 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CA12 belongs to the alpha-carbonic anhydrase family. It reversible hydration of carbon dioxide. Overexpression of the zinc enzyme CA12 is observed in certain human cancers. The structure reveals a prototypical CA fold; however, two CA12 domains associate to form an isologous dimer, an observation that is confirmed by studies of the enzyme in solution.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 CA12 antibody CL488-68224 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |