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验证数据展示

  • WB analysis using 66608-1-Ig

    WB analysis using 66608-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66608-1-Ig (CA3 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 66608-1-Ig

    IHC staining of mouse skeletal muscle using 66608-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66608-1-Ig (CA3 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 66608-1-Ig

    IHC staining of mouse skeletal muscle using 66608-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66608-1-Ig (CA3 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 66608-1-Ig

    WB analysis using 66608-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66608-1-Ig (CA3 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse skeletal muscle using 66608-1-Ig

    IF Staining of mouse skeletal muscle using 66608-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using CA3 antibody (66608-1-Ig, Clone: 3C10A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CA3 Monoclonal antibody

CA3 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66608-1-Ig

产品说明书

CloneNo. 3C10A2

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF-P, ELISA

别名

Carbonic anhydrase III, Car3, CA-III, CAIII, CA III

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCaki-1 cells, pig esophagus tissue, human skeletal muscle tissue, pig skeletal muscle tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue, rabbit skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse skeletal muscle tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66608-1-Ig targets CA3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 CA3 fusion protein Ag7513 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 carbonic anhydrase III, muscle specific
别名 Carbonic anhydrase III, Car3, CA-III, CAIII, CA III
计算分子量 29 kDa
观测分子量 29 kDa
GenBank蛋白编号BC004897
基因名称 CA3
Gene ID (NCBI) 761
RRIDAB_2881968
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP07451
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Carbonic anhydrase III (CA3), which belongs to the alpha-carbonic anhydrase family, is a cytoplasmic enzyme that exhibits a relatively low carbon dioxide hydratase activity. It is expressed at a very high level in skeletal muscle, where physical exercise has been shown to increase free radical production. In addition to its carbon dioxide hydratase activity, CA3 has been demonstrated to have a carboxyl esterase activity and phosphatase activity, which suggests that it is a tyrosine phosphatase (PMID: 10064618). CA3 was found to be localized in Type-I muscle fibers and could be used as a marker for abnormal Type-I muscle fibers in several neuromuscular diseases (PMID: 6221502).

实验方案

Product Specific Protocols
WB protocol for CA3 antibody 66608-1-IgDownload protocol
IHC protocol for CA3 antibody 66608-1-IgDownload protocol
IF protocol for CA3 antibody 66608-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols