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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66608-1-Ig (same clone as 66608-1-PBS)

    WB analysis using 66608-1-Ig (same clone as 66608-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66608-1-Ig (CA3 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66608-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 66608-1-Ig (same clone as 66608-1-PBS)

    IHC staining of mouse skeletal muscle using 66608-1-Ig (same clone as 66608-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66608-1-Ig (CA3 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66608-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 66608-1-Ig (same clone as 66608-1-PBS)

    IHC staining of mouse skeletal muscle using 66608-1-Ig (same clone as 66608-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66608-1-Ig (CA3 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66608-1-PBS in a different storage buffer formulation.

  • WB analysis using 66608-1-Ig (same clone as 66608-1-PBS)

    WB analysis using 66608-1-Ig (same clone as 66608-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66608-1-Ig (CA3 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66608-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse skeletal muscle using 66608-1-Ig (same clone as 66608-1-PBS)

    IF Staining of mouse skeletal muscle using 66608-1-Ig (same clone as 66608-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using CA3 antibody (66608-1-Ig, Clone: 3C10A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66608-1-PBS in a different storage buffer formulation.

CA3 Monoclonal antibody, PBS Only

CA3 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66608-1-PBS

产品说明书

CloneNo. 3C10A2

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF-P, Indirect ELISA

别名

Carbonic anhydrase III, Car3, CA-III, CAIII, CA III

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66608-1-PBS targets CA3 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 CA3 fusion protein Ag7513 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 carbonic anhydrase III, muscle specific
别名 Carbonic anhydrase III, Car3, CA-III, CAIII, CA III
计算分子量 29 kDa
观测分子量 29 kDa
GenBank蛋白编号BC004897
基因名称 CA3
Gene ID (NCBI) 761
RRIDAB_2881968
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP07451
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Carbonic anhydrase III (CA3), which belongs to the alpha-carbonic anhydrase family, is a cytoplasmic enzyme that exhibits a relatively low carbon dioxide hydratase activity. It is expressed at a very high level in skeletal muscle, where physical exercise has been shown to increase free radical production. In addition to its carbon dioxide hydratase activity, CA3 has been demonstrated to have a carboxyl esterase activity and phosphatase activity, which suggests that it is a tyrosine phosphatase (PMID: 10064618). CA3 was found to be localized in Type-I muscle fibers and could be used as a marker for abnormal Type-I muscle fibers in several neuromuscular diseases (PMID: 6221502).