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CA3 Monoclonal antibody, PBS Only

CA3 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse , Rat , Pig

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C10A2

Cat No : 66608-1-PBS

Print datasheet

Synonyms

CA III, CA3, CAIII, Car3, Carbonate dehydratase III, Carbonic anhydrase 3, Carbonic anhydrase III

验证数据展示

  • WB analysis using 66608-1-Ig (same clone as 66608-1-PBS)

    WB analysis using 66608-1-Ig (same clone as 66608-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66608-1-Ig (CA3 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66608-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 66608-1-Ig (same clone as 66608-1-PBS)

    IHC staining of mouse skeletal muscle using 66608-1-Ig (same clone as 66608-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66608-1-Ig (CA3 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66608-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 66608-1-Ig (same clone as 66608-1-PBS)

    IHC staining of mouse skeletal muscle using 66608-1-Ig (same clone as 66608-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66608-1-Ig (CA3 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66608-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse skeletal muscle using 66608-1-Ig (same clone as 66608-1-PBS)

    IF Staining of mouse skeletal muscle using 66608-1-Ig (same clone as 66608-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using CA3 antibody (66608-1-Ig, Clone: 3C10A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66608-1-PBS in a different storage buffer formulation.

产品信息

66608-1-PBS targets CA3 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse , Rat , Pig samples.

Tested Applications WB, IF, IHC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse , Rat , Pig
Immunogen CA3 fusion protein Ag7513 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name carbonic anhydrase III, muscle specific
Synonyms CA III, CA3, CAIII, Car3, Carbonate dehydratase III, Carbonic anhydrase 3, Carbonic anhydrase III
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC004897
Gene Symbol CA3
Gene ID (NCBI) 761
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07451
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Carbonic anhydrase III (CA3), which belongs to the alpha-carbonic anhydrase family, is a cytoplasmic enzyme that exhibits a relatively low carbon dioxide hydratase activity. It is expressed at a very high level in skeletal muscle, where physical exercise has been shown to increase free radical production. In addition to its carbon dioxide hydratase activity, CA3 has been demonstrated to have a carboxyl esterase activity and phosphatase activity, which suggests that it is a tyrosine phosphatase (PMID: 10064618). CA3 was found to be localized in Type-I muscle fibers and could be used as a marker for abnormal Type-I muscle fibers in several neuromuscular diseases (PMID: 6221502).