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验证数据展示

  • IF Staining of mouse skeletal muscle using CL488-15197

    IF Staining of mouse skeletal muscle using CL488-15197

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using CoraLite® Plus 488 CA3 antibody (CL488-15197) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  • KD/KO验证

CoraLite® Plus 488-conjugated CA3 Polyclonal antibody

CA3 Polyclonal Antibody for IF-P
Cat No. CL488-15197

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF-P

别名

CA III, CA3, CAIII, Car3, Carbonate dehydratase III, Carbonic anhydrase 3, Carbonic anhydrase III

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse skeletal muscle tissue
For other applications, we recommend the unconjugated version of this antibody, 15197-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-15197 targets CA3 in IF-P applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse, rat
免疫原 CA3 fusion protein Ag7344 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 carbonic anhydrase III, muscle specific
别名 CA III, CA3, CAIII, Car3, Carbonate dehydratase III, Carbonic anhydrase 3, Carbonic anhydrase III
计算分子量 29 kDa
观测分子量 30 kDa
GenBank蛋白编号BC004897
基因名称 CA3
Gene ID (NCBI) 761
RRIDAB_3672623
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP07451
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Carbonic anhydrase III (CA3), which belongs to the alpha-carbonic anhydrase family, is a cytoplasmic enzyme that exhibits a relatively low carbon dioxide hydratase activity. It is expressed at a very high level in skeletal muscle, where physical exercise has been shown to increase free radical production. In addition to its carbon dioxide hydratase activity, CA3 has been demonstrated to have a carboxyl esterase activity and phosphatase activity, which suggests that it is a tyrosine phosphatase (PMID: 10064618). CA3 was found to be localized in Type-I muscle fibers and could be used as a marker for abnormal Type-I muscle fibers in several neuromuscular diseases (PMID: 6221502).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CA3 antibody CL488-15197Download protocol
Standard Protocols
Click here to view our Standard Protocols