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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CALD1 Monoclonal antibody

CALD1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E1C11

Cat no : CL488-66693

Print datasheet

Synonyms

Caldesmon, CDM, caldesmon 1, CAD, 1E1C11

验证数据展示

  • IF Staining of HepG2 using CL488-66693

    IF Staining of HepG2 using CL488-66693

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL488-66693 (CALD1 antibody) at dilution of 1:100.

  • FC experiment of HeLa using CL488-66693

    FC experiment of HeLa using CL488-66693

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human CALD1 (CL488-66693, Clone:1E1C11) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2b Isotype Control (MPC-11) (CL488-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CALD1 Ready-To-Use IHC Kit. CALD1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66693-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66693 targets CALD1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen CALD1 fusion protein Ag13676 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name caldesmon 1
Synonyms Caldesmon, CDM, caldesmon 1, CAD, 1E1C11
Calculated Molecular Weight 538 aa, 63 kDa
Observed Molecular Weight 70 kDa~75 kDa
GenBank Accession NumberBC040354
Gene Symbol CALD1
Gene ID (NCBI) 800
RRIDAB_2883368
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ05682
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CALD1 antibody CL488-66693Download protocol
Standard Protocols
Click here to view our Standard Protocols