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CALML3 Polyclonal antibody

CALML3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 17275-1-AP

Print datasheet

Synonyms

CALML3, calmodulin like 3, Calmodulin like protein 3, CaM like protein, CLP

验证数据展示

  • WB analysis using 17275-1-AP

    WB analysis using 17275-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 17275-1-AP (CALML3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 17275-1-AP

    IHC staining of human liver cancer using 17275-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17275-1-AP (CALML3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 17275-1-AP

    IHC staining of human liver cancer using 17275-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17275-1-AP (CALML3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of A431 using 17275-1-AP

    IP experiment of A431 using 17275-1-AP

    IP result of anti-CALML3 (IP:17275-1-AP, 4ug; Detection:17275-1-AP 1:300) with A431 cells lysate 2400 ug.

  • IF Staining of A431 using 17275-1-AP

    IF Staining of A431 using 17275-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CALML3 antibody (17275-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inA431 cells
Positive IP detected inA431 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17275-1-AP targets CALML3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human
Immunogen CALML3 fusion protein Ag11037 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name calmodulin-like 3
Synonyms CALML3, calmodulin like 3, Calmodulin like protein 3, CaM like protein, CLP
Calculated Molecular Weight 149 aa, 17 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberBC031889
Gene Symbol CALML3
Gene ID (NCBI) 810
RRIDAB_2878374
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP27482
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CALML3 antibody 17275-1-APDownload protocol
IHC protocol for CALML3 antibody 17275-1-APDownload protocol
IF protocol for CALML3 antibody 17275-1-APDownload protocol
IP protocol for CALML3 antibody 17275-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols