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CoraLite® Plus 488-conjugated CALML3 Polyclonal antibody

CALML3 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-17275

Print datasheet

Synonyms

CALML3, calmodulin like 3, Calmodulin like protein 3, CaM like protein, CLP

验证数据展示

  • IF Staining of A431 using CL488-17275

    IF Staining of A431 using CL488-17275

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using CoraLite® Plus 488 CALML3 antibody (CL488-17275) at dilution of 1:100.

  • FC experiment of A431 using CL488-17275

    FC experiment of A431 using CL488-17275

    1x10^6 A431 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human CALML3 (CL488-17275)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells
Positive FC detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 17275-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-17275 targets CALML3 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen CALML3 fusion protein Ag11037 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name calmodulin-like 3
Synonyms CALML3, calmodulin like 3, Calmodulin like protein 3, CaM like protein, CLP
Calculated Molecular Weight 149 aa, 17 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberBC031889
Gene Symbol CALML3
Gene ID (NCBI) 810
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP27482
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CALML3 antibody CL488-17275Download protocol
FC protocol for CL Plus 488 CALML3 antibody CL488-17275Download protocol
Standard Protocols
Click here to view our Standard Protocols