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CoraLite® Plus 488-conjugated CAMK2 Monoclonal antibody

CAMK2 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E3B3

Cat no : CL488-66843

Print datasheet

Synonyms

CaM kinase II subunit alpha, CaMK II subunit alpha, CAMK2A, CAMKA, KIAA0968

验证数据展示

  • WB analysis using CL488-66843

    WB analysis using CL488-66843

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66843 (CAMK2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inrat brain tissue, mouse brain tissue
For other applications, we recommend the unconjugated version of this antibody, 66843-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66843 targets CAMK2 in WB applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications WB Application Description
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen CAMK2 fusion protein Ag4736 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name calcium/calmodulin-dependent protein kinase II alpha
Synonyms CaM kinase II subunit alpha, CaMK II subunit alpha, CAMK2A, CAMKA, KIAA0968
Calculated Molecular Weight 478 aa, 54 kDa
Observed Molecular Weight 45-60 kDa
GenBank Accession NumberBC040457
Gene Symbol CaMKII alpha
Gene ID (NCBI) 815
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UQM7
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. It is a member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. This antibody can recognize all the members of CAMK2 family.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 CAMK2 antibody CL488-66843Download protocol
Standard Protocols
Click here to view our Standard Protocols