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验证数据展示

  • IHC staining of mouse brain using 81014-1-RR (same clone as 81014-1-PBS)

    IHC staining of mouse brain using 81014-1-RR (same clone as 81014-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81014-1-RR (CAMK2D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81014-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CAMK2D antibody included
  • WB analysis using 81014-1-RR (same clone as 81014-1-PBS)

    WB analysis using 81014-1-RR (same clone as 81014-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81014-1-RR (CAMK2D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81014-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81014-1-RR

    Affinity and Kinetic Characterization of 81014-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81014-1-RR against Human CAMK2D were performed. The affinity constant is 81.6 nM.

CAMK2D Recombinant antibody, PBS Only

CAMK2D Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 81014-1-PBS

产品说明书

CloneNo. 1M10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, Indirect ELISA

别名

CaMKII delta, EC:2.7.11.17, CaMK-II subunit delta, CaM kinase II subunit delta, Calcium/calmodulin-dependent protein kinase type II subunit delta

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CAMK2D Ready-To-Use IHC Kit. CAMK2D primary antibody included.

产品信息

81014-1-PBS targets CAMK2D in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 CAMK2D fusion protein Ag31276 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 calcium/calmodulin-dependent protein kinase II delta
别名 CaMKII delta, EC:2.7.11.17, CaMK-II subunit delta, CaM kinase II subunit delta, Calcium/calmodulin-dependent protein kinase type II subunit delta
计算分子量 59 kDa
观测分子量54-59 kDa
GenBank蛋白编号BC032784
基因名称 CAMK2D
Gene ID (NCBI) 817
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13557
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CaMKII delta, also named as CAMKD, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and CaMK subfamily. CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release, composed of four different chains: alpha, beta, gamma, and delta. The CAMK2 protein kinases contain a unique C-terminal subunit association domain responsible for oligomerization. The different chains assemble into homo-or heteromultimeric holoenzymes composed of 8 to 12 subunits. This antibody is specific to the C-term of delta chain, CaMKII delta. It may also recognize CAMK2A due to the high homology.