验证数据展示
经过测试的应用
| Positive WB detected in | rat brain tissue, mouse brain tissue, pig brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
81014-1-RR targets CAMK2D in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat, pig |
| 免疫原 | CAMK2D fusion protein Ag31276 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | calcium/calmodulin-dependent protein kinase II delta |
| 别名 | CaMKII delta, EC:2.7.11.17, CaMK-II subunit delta, CaM kinase II subunit delta, Calcium/calmodulin-dependent protein kinase type II subunit delta |
| 计算分子量 | 59 kDa |
| 观测分子量 | 54-59 kDa |
| GenBank蛋白编号 | BC032784 |
| 基因名称 | CAMK2D |
| Gene ID (NCBI) | 817 |
| RRID | AB_2923696 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q13557 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CaMKII delta, also named as CAMKD, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and CaMK subfamily. CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release, composed of four different chains: alpha, beta, gamma, and delta. The CAMK2 protein kinases contain a unique C-terminal subunit association domain responsible for oligomerization. The different chains assemble into homo-or heteromultimeric holoenzymes composed of 8 to 12 subunits. This antibody is specific to the C-term of delta chain, CaMKII delta. It may also recognize CAMK2A due to the high homology.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CAMK2D antibody 81014-1-RR | Download protocol |
| IHC protocol for CAMK2D antibody 81014-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |