验证数据展示
经过测试的应用
| Positive WB detected in | rat brain tissue, mouse brain tissue, rat thymus tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68452-1-Ig targets CAMK4 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, mouse, rat |
| 免疫原 | CAMK4 fusion protein Ag33332 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | calcium/calmodulin-dependent protein kinase IV |
| 别名 | CaM kinase GR, CaMK GR, CaMK IV, CAMK4 |
| 计算分子量 | 473 aa, 52 kDa |
| 观测分子量 | 56-65 kDa |
| GenBank蛋白编号 | BC025687 |
| 基因名称 | CAMK4 |
| Gene ID (NCBI) | 814 |
| RRID | AB_3085164 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q16566 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CaMK4 (Calcium/calmodulin-dependent protein kinase IV) is a multifunctional serine/threonine kinase that regulates gene expression by activating transcription factors in a wide range of immune cells including T cells and antigen-presenting cells. The function of CaMK4 is suggested to be abnormal mainly in systemic lupus erythematosus (SLE), which is characterized by autoantibody production, immune complex formation, and immune dysregulation (PMID: 29252071). CaMKIV can translocate between the nucleus and cytoplasm (PMID: 21301225)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CAMK4 antibody 68452-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |