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CAMKK1 Polyclonal antibody

CAMKK1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13624-1-AP

Print datasheet

Synonyms

CaM KK 1, CaM kinase kinase alpha, CaM kinase kinase 1, CaM kinase IV kinase, Calcium/calmodulin-dependent protein kinase kinase alpha

验证数据展示

  • WB analysis of Jurkat using 13624-1-AP

    WB analysis of Jurkat using 13624-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13624-1-AP (CAMKK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 13624-1-AP

    IHC staining of human stomach using 13624-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13624-1-AP (CAMKK1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of Jurkat using 13624-1-AP

    IP experiment of Jurkat using 13624-1-AP

    IP result of anti-CAMKK1 (IP:13624-1-AP, 4ug; Detection:13624-1-AP 1:1000) with Jurkat cells lysate 4000ug.

  • IHC staining of human stomach using 13624-1-AP

    IHC staining of human stomach using 13624-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13624-1-AP (CAMKK1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inJurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13624-1-AP targets CAMKK1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CAMKK1 fusion protein Ag4534 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name calcium/calmodulin-dependent protein kinase kinase 1, alpha
Synonyms CaM KK 1, CaM kinase kinase alpha, CaM kinase kinase 1, CaM kinase IV kinase, Calcium/calmodulin-dependent protein kinase kinase alpha
Calculated Molecular Weight 520 aa, 57 kDa
Observed Molecular Weight 63 kDa
GenBank Accession NumberBC031647
Gene Symbol CAMKK1
Gene ID (NCBI) 84254
RRIDAB_2068403
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N5S9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calcium/calmodulin-dependent protein kinase kinase 1 catalyzes the phosphorylation and activation of Ca(2+)/calmodulin kinase (CaMK) as an upstream component of the CaMK cascade that has been implicated in neuronal gene transcription, synaptic plasticity, and long-term memory consolidation .CAMKK1 has two isoforms of 56kda and 58kda and involved in regulating cell apoptosis, promotes cell survival by phosphorylating AKT1/PKB that inhibits pro-apoptotic BAD/Bcl2-antagonist of cell death .Researches showed that Camkk1 plays a selective role in contextual fear memory.This 66-68kda protein can be phosphorylated(probably antophosphorylation)(PMID:7961813).This antibody recognize the N-terminal of CAMKK1 .

实验方案

Product Specific Protocols
WB protocol for CAMKK1 antibody 13624-1-APDownload protocol
IHC protocol for CAMKK1 antibody 13624-1-APDownload protocol
IP protocol for CAMKK1 antibody 13624-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols