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验证数据展示

  • WB analysis of Jurkat using 13624-1-AP

    WB analysis of Jurkat using 13624-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13624-1-AP (CAMKK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 13624-1-AP

    IHC staining of human stomach using 13624-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13624-1-AP (CAMKK1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CAMKK1 antibody included
  • IP experiment of Jurkat using 13624-1-AP

    IP experiment of Jurkat using 13624-1-AP

    IP result of anti-CAMKK1 (IP:13624-1-AP, 4ug; Detection:13624-1-AP 1:1000) with Jurkat cells lysate 4000ug.

  • IHC staining of human stomach using 13624-1-AP

    IHC staining of human stomach using 13624-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13624-1-AP (CAMKK1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CAMKK1 antibody included

CAMKK1 Polyclonal antibody

CAMKK1 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 13624-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

CaM KK 1, CaM kinase kinase alpha, CaM kinase kinase 1, CaM kinase IV kinase, Calcium/calmodulin-dependent protein kinase kinase alpha

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CAMKK1 Ready-To-Use IHC Kit. CAMKK1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13624-1-AP targets CAMKK1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CAMKK1 fusion protein Ag4534 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 calcium/calmodulin-dependent protein kinase kinase 1, alpha
别名 CaM KK 1, CaM kinase kinase alpha, CaM kinase kinase 1, CaM kinase IV kinase, Calcium/calmodulin-dependent protein kinase kinase alpha
计算分子量 520 aa, 57 kDa
观测分子量 63 kDa
GenBank蛋白编号BC031647
基因名称 CAMKK1
Gene ID (NCBI) 84254
RRIDAB_2068403
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N5S9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calcium/calmodulin-dependent protein kinase kinase 1 catalyzes the phosphorylation and activation of Ca(2+)/calmodulin kinase (CaMK) as an upstream component of the CaMK cascade that has been implicated in neuronal gene transcription, synaptic plasticity, and long-term memory consolidation .CAMKK1 has two isoforms of 56kda and 58kda and involved in regulating cell apoptosis, promotes cell survival by phosphorylating AKT1/PKB that inhibits pro-apoptotic BAD/Bcl2-antagonist of cell death .Researches showed that Camkk1 plays a selective role in contextual fear memory.This 66-68kda protein can be phosphorylated(probably antophosphorylation)(PMID:7961813).This antibody recognize the N-terminal of CAMKK1 .

实验方案

Product Specific Protocols
WB protocol for CAMKK1 antibody 13624-1-APDownload protocol
IHC protocol for CAMKK1 antibody 13624-1-APDownload protocol
IP protocol for CAMKK1 antibody 13624-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols