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验证数据展示

  • FC experiment of MCF-7 using 82940-2-RR

    FC experiment of MCF-7 using 82940-2-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Anti-Human CAMP (82940-2-RR, Clone:230157C6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of Hela using 82940-2-RR

    FC experiment of Hela using 82940-2-RR

    (blue)1x10^6 Hela cells were intracellularly stained with 0.25 ug Anti-Human CAMP (82940-2-RR, Clone:230157C6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution (red), or 0.25 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82940-2-RR

    Affinity and Kinetic Characterization of 82940-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82940-2-RR against Human CAMP were performed. The affinity constant is 1.50 nM.

CAMP Recombinant antibody

CAMP Uni-rAbTM Recombinant Antibody for FC (Intra), ELISA
Cat No. 82940-2-RR

产品说明书

CloneNo. 230157C6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra), ELISA

别名

CAP18, Antibacterial peptide LL-23, Antibacterial peptide KS-30, Antibacterial peptide KR-20, Antibacterial peptide FF-33

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inMCF-7 cells, Hela cells

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82940-2-RR targets CAMP in FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 CAMP fusion protein Ag2622 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cathelicidin antimicrobial peptide
别名 CAP18, Antibacterial peptide LL-23, Antibacterial peptide KS-30, Antibacterial peptide KR-20, Antibacterial peptide FF-33
计算分子量 170 aa, 19 kDa
GenBank蛋白编号BC055089
基因名称 CAMP
Gene ID (NCBI) 820
RRIDAB_3670683
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP49913
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CAMP is a member of an antimicrobial peptide family, characterized by a highly conserved N-terminal signal peptide containing a cathelin domain and a structurally variable cationic antimicrobial peptide, which is produced by extracellular proteolysis from the C-terminus. In addition to its antibacterial, antifungal, and antiviral activities, the encoded protein functions in cell chemotaxis, immune mediator induction, and inflammatory response regulation. CAMP encodes the 18-kDa proprotein hCAP18. FALL-39 and LL-37 are the mature cathelicidin peptides. This antibody can recognize all the proprotein and cleaved species.

实验方案

Product Specific Protocols
FC protocol for CAMP antibody 82940-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols