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CAP1 Monoclonal antibody, PBS Only

CAP1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H3A10

Cat No : 68207-1-PBS

Print datasheet

Synonyms

CAP, CAP 1, CAP1, CAP1 PEN

验证数据展示

  • WB analysis using 68207-1-Ig (same clone as 68207-1-PBS)

    WB analysis using 68207-1-Ig (same clone as 68207-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68207-1-Ig (CAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 68207-1-Ig (same clone as 68207-1-PBS)

    IF Staining of HepG2 using 68207-1-Ig (same clone as 68207-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CAP1 antibody (68207-1-Ig, Clone: 2H3A10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 68207-1-Ig (same clone as 68207-1-PBS)

    IF Staining of HepG2 using 68207-1-Ig (same clone as 68207-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CAP1 antibody (68207-1-Ig, Clone: 2H3A10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.

  • WB analysis of pig lung using 68207-1-Ig (same clone as 68207-1-PBS)

    WB analysis of pig lung using 68207-1-Ig (same clone as 68207-1-PBS)

    pig lung tissue were subjected to SDS PAGE followed by western blot with 68207-1-Ig (CAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.

  • WB analysis of human placenta using 68207-1-Ig (same clone as 68207-1-PBS)

    WB analysis of human placenta using 68207-1-Ig (same clone as 68207-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 68207-1-Ig (CAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 68207-1-Ig (same clone as 68207-1-PBS)

    IHC staining of human colon cancer using 68207-1-Ig (same clone as 68207-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68207-1-Ig (CAP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CAP1 antibody included
  • IHC staining of mouse testis using 68207-1-Ig (same clone as 68207-1-PBS)

    IHC staining of mouse testis using 68207-1-Ig (same clone as 68207-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68207-1-Ig (CAP1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CAP1 antibody included
  • IF Staining of HeLa using 68207-1-Ig (same clone as 68207-1-PBS)

    IF Staining of HeLa using 68207-1-Ig (same clone as 68207-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CAP1 antibody (68207-1-Ig, Clone: 2H3A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CAP1 Ready-To-Use IHC Kit. CAP1 primary antibody included.

产品信息

68207-1-PBS targets CAP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat, pig, rabbit
Immunogen CAP1 fusion protein Ag8876 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CAP, adenylate cyclase-associated protein 1 (yeast)
Synonyms CAP, CAP 1, CAP1, CAP1 PEN
Calculated Molecular Weight 475 aa, 52 kDa
Observed Molecular Weight52 kDa
GenBank Accession NumberBC013963
Gene Symbol CAP1
Gene ID (NCBI) 10487
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ01518
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CAP1, also known as CAP, CAP1-PEN, belongs to the CAP family and contains 1 C-CAP/cofactor C-like domain. CAP1 binds to monomeric actin and regulates the actin cytoskeleton to control cell size, adhesion, and migration (PMID: 23737525). CAP1 also functions as a receptor for inflammatory cytokine resistin (PMID: 24606903). CAP1 is an abundant and ubiquitously expressed protein. Western blot analysis detected CAP1 at an apparent molecular mass of 52 kDa.