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验证数据展示

  • IF Staining of HepG2 using CL488-68207

    IF Staining of HepG2 using CL488-68207

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 CAP1 antibody (CL488-68207, Clone: 2H3A10 ) at dilution of 1:200.

  • IF Staining of HepG2 using CL488-68207

    IF Staining of HepG2 using CL488-68207

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 CAP1 antibody (CL488-68207, Clone: 2H3A10 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated CAP1 Monoclonal antibody

CAP1 Monoclonal Antibody for IF/ICC
Cat No. CL488-68207

产品说明书

CloneNo. 2H3A10

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat, pig, rabbit

应用

IF/ICC

别名

CAP, CAP 1, CAP1, CAP1 PEN

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CAP1 Ready-To-Use IHC Kit. CAP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68207-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68207 targets CAP1 in IF/ICC applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, mouse, rat, pig, rabbit
免疫原 CAP1 fusion protein Ag8876 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 CAP, adenylate cyclase-associated protein 1 (yeast)
别名 CAP, CAP 1, CAP1, CAP1 PEN
计算分子量 475 aa, 52 kDa
观测分子量52 kDa
GenBank蛋白编号BC013963
基因名称 CAP1
Gene ID (NCBI) 10487
RRIDAB_3084441
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ01518
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CAP1, also known as CAP, CAP1-PEN, belongs to the CAP family and contains 1 C-CAP/cofactor C-like domain. CAP1 binds to monomeric actin and regulates the actin cytoskeleton to control cell size, adhesion, and migration (PMID: 23737525). CAP1 also functions as a receptor for inflammatory cytokine resistin (PMID: 24606903). CAP1 is an abundant and ubiquitously expressed protein. Western blot analysis detected CAP1 at an apparent molecular mass of 52 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CAP1 antibody CL488-68207Download protocol
FC protocol for CL Plus 488 CAP1 antibody CL488-68207Download protocol
Standard Protocols
Click here to view our Standard Protocols