CAPN3 Monoclonal antibody
CAPN3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig
Applications
WB, IHC, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
1G12A5
验证数据展示
经过测试的应用
Positive WB detected in | human skeletal muscle tissue, pig skeletal muscle tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue |
Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | U2OS cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
IP | See 1 publications below |
产品信息
67366-1-Ig targets CAPN3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Cited Applications | WB, IP |
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human |
Immunogen | CAPN3 fusion protein Ag13179 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | calpain 3, (p94) |
Synonyms | Calpain p94, Calpain L3, calpain 3, (p94), Calpain 3, Calcium-activated neutral proteinase 3 |
Calculated Molecular Weight | 18 kDa |
Observed Molecular Weight | 90-105 kDa, 55-60 kDa, 30-35 kDa |
GenBank Accession Number | BC004883 |
Gene Symbol | CAPN3 |
Gene ID (NCBI) | 825 |
RRID | AB_2882618 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P20807 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for CAPN3 antibody 67366-1-Ig | Download protocol |
IHC protocol for CAPN3 antibody 67366-1-Ig | Download protocol |
IF protocol for CAPN3 antibody 67366-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Ther Engineered mischarged transfer RNAs for correcting pathogenic missense mutations |