CAPN3 Monoclonal antibody

CAPN3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

1G12A5

Cat no : 67366-1-Ig

Print datasheet

Synonyms

Calpain p94, Calpain L3, calpain 3, (p94), Calpain 3, Calcium-activated neutral proteinase 3



经过测试的应用

Positive WB detected inhuman skeletal muscle tissue, pig skeletal muscle tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy CAPN3 Ready-To-Use IHC Kit. CAPN3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67366-1-Ig targets CAPN3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IP
Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman
Immunogen CAPN3 fusion protein Ag13179 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name calpain 3, (p94)
Synonyms Calpain p94, Calpain L3, calpain 3, (p94), Calpain 3, Calcium-activated neutral proteinase 3
Calculated Molecular Weight 18 kDa
Observed Molecular Weight 90-105 kDa, 55-60 kDa, 30-35 kDa
GenBank Accession NumberBC004883
Gene Symbol CAPN3
Gene ID (NCBI) 825
RRIDAB_2882618
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP20807
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calpain 3 (CAPN3), a major intracellular protease, is a muscle-specific member of the calpain large subunit family that specifically binds to titin. CAPN3 plays a role in the dysferlin protein complex and that disruption of CAPN3 function may affect muscle membrane repair and remodeling. CAPN3 has some isoforms with MW of 94, 84, 93, 36, 18 kDa. CAPN3 autolysis generates a small N-terminal fragment of 34 kDa and a large C-terminal fragment whose size ranges from 55 to 60 kDa during self-processing (PMID: 9794799, 14645524).

实验方案

Product Specific Protocols
WB protocol for CAPN3 antibody 67366-1-IgDownload protocol
IHC protocol for CAPN3 antibody 67366-1-IgDownload protocol
IF protocol for CAPN3 antibody 67366-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

Mol Ther

Engineered mischarged transfer RNAs for correcting pathogenic missense mutations

Authors - Yichen Hou