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CAPN3 Monoclonal antibody, PBS Only

CAPN3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1G12A5

Cat no : 67366-1-PBS

Print datasheet

Synonyms

Calpain p94, Calpain L3, calpain 3, (p94), Calpain 3, Calcium-activated neutral proteinase 3

验证数据展示

  • IF Staining of U2OS using 67366-1-Ig (same clone as 67366-1-PBS)

    IF Staining of U2OS using 67366-1-Ig (same clone as 67366-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CAPN3 antibody (67366-1-Ig, Clone: 1G12A5 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 67366-1-PBS in a different storage buffer formulation.

  • WB analysis using 67366-1-Ig (same clone as 67366-1-PBS)

    WB analysis using 67366-1-Ig (same clone as 67366-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67366-1-Ig (CAPN3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67366-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 67366-1-Ig (same clone as 67366-1-PBS)

    IHC staining of mouse skeletal muscle using 67366-1-Ig (same clone as 67366-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67366-1-Ig (CAPN3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67366-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CAPN3 antibody included
  • IHC staining of mouse skeletal muscle using 67366-1-Ig (same clone as 67366-1-PBS)

    IHC staining of mouse skeletal muscle using 67366-1-Ig (same clone as 67366-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67366-1-Ig (CAPN3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67366-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CAPN3 antibody included
Planning an IHC experiment? We recommend our IHCeasy CAPN3 Ready-To-Use IHC Kit. CAPN3 primary antibody included.

产品信息

67366-1-PBS targets CAPN3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen CAPN3 fusion protein Ag13179 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name calpain 3, (p94)
Synonyms Calpain p94, Calpain L3, calpain 3, (p94), Calpain 3, Calcium-activated neutral proteinase 3
Calculated Molecular Weight 18 kDa
Observed Molecular Weight 90-105 kDa, 55-60 kDa, 30-35 kDa
GenBank Accession NumberBC004883
Gene Symbol CAPN3
Gene ID (NCBI) 825
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP20807
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Calpain 3 (CAPN3), a major intracellular protease, is a muscle-specific member of the calpain large subunit family that specifically binds to titin. CAPN3 plays a role in the dysferlin protein complex and that disruption of CAPN3 function may affect muscle membrane repair and remodeling. CAPN3 has some isoforms with MW of 94, 84, 93, 36, 18 kDa. CAPN3 autolysis generates a small N-terminal fragment of 34 kDa and a large C-terminal fragment whose size ranges from 55 to 60 kDa during self-processing (PMID: 9794799, 14645524).