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验证数据展示

  • IF Staining of HeLa using CL555-66352

    IF Staining of HeLa using CL555-66352

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®555 CAPRIN1 antibody (CL555-66352, Clone: 6B10F11 ) at dilution of 1:200.

CoraLite®555-conjugated CAPRIN1 Monoclonal antibody

CAPRIN1 Monoclonal Antibody for IF/ICC
Cat No. CL555-66352

Print datasheet

CloneNo. 6B10F11

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Synonyms

caprin 1, CAPRIN1, GPI anchored protein p137, GPI p137, GPIAP1, GPIP137, M11S1, p137GPI

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®555
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CAPRIN1 Ready-To-Use IHC Kit. CAPRIN1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66352-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-66352 targets CAPRIN1 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen CAPRIN1 fusion protein Ag7413 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cell cycle associated protein 1
Synonyms caprin 1, CAPRIN1, GPI anchored protein p137, GPI p137, GPIAP1, GPIP137, M11S1, p137GPI
Calculated Molecular Weight 78 kDa
Observed Molecular Weight 116 kDa
GenBank Accession NumberBC001731
Gene Symbol CAPRIN1
Gene ID (NCBI) 4076
RRIDAB_2923896
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ14444
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CAPRIN1(Caprin-1) is a cytoplasmic phosphoprotein which may regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types. Its levels, except in the brain, are tightly correlated with cellular proliferation. Suppression of expression of human Caprin-1 resulted in slowing of the proliferation rate,which suggests its essential for normal cellular proliferation.

实验方案

Product Specific Protocols
IF protocol for CL555 CAPRIN1 antibody CL555-66352Download protocol
Standard Protocols
Click here to view our Standard Protocols