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CAPSL Polyclonal antibody

CAPSL Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17174-1-AP

Print datasheet

Synonyms

Calcyphosin like protein, calcyphosine like, CAPSL

验证数据展示

  • WB analysis of mouse lung using 17174-1-AP

    WB analysis of mouse lung using 17174-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 17174-1-AP (CAPSL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 17174-1-AP

    IHC staining of human lung cancer using 17174-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 17174-1-AP (CAPSL antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human lung cancer using 17174-1-AP

    IHC staining of human lung cancer using 17174-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 17174-1-AP (CAPSL antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of mouse lung using 17174-1-AP

    IP experiment of mouse lung using 17174-1-AP

    IP result of anti-CAPSL (IP:17174-1-AP, 4ug; Detection:17174-1-AP 1:500) with mouse lung tissue lysate 4000ug.

经过测试的应用

Positive WB detected inmouse lung tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

17174-1-AP targets CAPSL in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen CAPSL fusion protein Ag10771 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name calcyphosine-like
Synonyms Calcyphosin like protein, calcyphosine like, CAPSL
Calculated Molecular Weight 198 aa, 23 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC017586
Gene Symbol CAPSL
Gene ID (NCBI) 133690
RRIDAB_2070031
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WWF8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CAPSL antibody 17174-1-APDownload protocol
IHC protocol for CAPSL antibody 17174-1-APDownload protocol
IP protocol for CAPSL antibody 17174-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Sci Rep

Calcyphosine-like (CAPSL) is regulated in Multiple Symmetric Lipomatosis and is involved in Adipogenesis.

Authors - Angie Lindner
View Article