验证数据展示
经过测试的应用
| Positive IF/ICC detected in | MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy CAPZA1 Ready-To-Use IHC Kit. CAPZA1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66066-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66066 targets CAPZA1 in IF/ICC applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human |
| 免疫原 | CAPZA1 fusion protein Ag18841 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | capping protein (actin filament) muscle Z-line, alpha 1 |
| 别名 | CAPPA1, CAPZ, CapZ alpha 1, CAPZA1, CAZ1 |
| 计算分子量 | 286 aa, 33 kDa |
| GenBank蛋白编号 | BC000144 |
| 基因名称 | CAPZA1 |
| Gene ID (NCBI) | 829 |
| RRID | AB_2883248 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P52907 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CAPZA1 (F-actin-capping protein subunit alpha-1) is a member of the F-actin capping protein alpha subunit family and regulates actin filament growth, stability and disassembly. Differential expression of CAPZA1 has been found in gastric cancer and melanoma, indicating its possible role in tumorigenesis. (21566537, 23545944)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CAPZA1 antibody CL488-66066 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |