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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CAPZA1 Monoclonal antibody

CAPZA1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

10H1F9

Cat no : CL488-66066

Print datasheet

Synonyms

CAPPA1, CAPZ, CapZ alpha 1, CAPZA1, CAZ1

验证数据展示

  • IF Staining of MCF-7 using CL488-66066

    IF Staining of MCF-7 using CL488-66066

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CL488-66066 (CAPZA1 antibody) at dilution of 1:100.

经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy CAPZA1 Ready-To-Use IHC Kit. CAPZA1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66066-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL488-66066 is CAPZA1 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen CAPZA1 fusion protein Ag18841 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name capping protein (actin filament) muscle Z-line, alpha 1
Synonyms CAPPA1, CAPZ, CapZ alpha 1, CAPZA1, CAZ1
Calculated Molecular Weight 286 aa, 33 kDa
GenBank Accession NumberBC000144
Gene Symbol CAPZA1
Gene ID (NCBI) 829
RRIDAB_2883248
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP52907
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CAPZA1 (F-actin-capping protein subunit alpha-1) is a member of the F-actin capping protein alpha subunit family and regulates actin filament growth, stability and disassembly. Differential expression of CAPZA1 has been found in gastric cancer and melanoma, indicating its possible role in tumorigenesis. (21566537, 23545944)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CAPZA1 antibody CL488-66066Download protocol
Standard Protocols
Click here to view our Standard Protocols