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CARD11 Monoclonal antibody, PBS Only

CARD11 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3F5H6

Cat no : 68269-1-PBS

Print datasheet

Synonyms

BIMP3, CARD11, Carma 1, CARMA1

验证数据展示

  • WB analysis using 68269-1-Ig (same clone as 68269-1-PBS)

    WB analysis using 68269-1-Ig (same clone as 68269-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68269-1-Ig (CARD11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68269-1-PBS in a different storage buffer formulation.

  • IF Staining of L02 using 68269-1-Ig (same clone as 68269-1-PBS)

    IF Staining of L02 using 68269-1-Ig (same clone as 68269-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using CARD11 antibody (68269-1-Ig, Clone: 3F5H6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68269-1-PBS in a different storage buffer formulation.

产品信息

68269-1-PBS targets CARD11 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen CARD11 fusion protein Ag16468 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name caspase recruitment domain family, member 11
Synonyms BIMP3, CARD11, Carma 1, CARMA1
Calculated Molecular Weight 1154 aa, 133 kDa
Observed Molecular Weight 140 kDa
GenBank Accession NumberBC111719
Gene Symbol CARD11
Gene ID (NCBI) 84433
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BXL7
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CARD11 also named as ,BIMP3 or CARMA1, is a 1154 amino acid protein, which contains one PDZ domain, one guanylate kinase-like domain, and one CARD domain. CARD11 localizes in the cytoplasm and co-localized with DPP4 in membrane raft. CARD11 is detected in adult peripheral blood leukocytes, thymus, spleen and liver. CARD11 also is found in CARD11 promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K-562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. CARD11 is involved in the co-stimulatory signal essential for T-cell receptor mediated T-cell activation.