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验证数据展示

  • WB analysis using 68269-1-Ig

    WB analysis using 68269-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68269-1-Ig (CARD11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of L02 using 68269-1-Ig

    IF Staining of L02 using 68269-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using CARD11 antibody (68269-1-Ig, Clone: 3F5H6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

CARD11 Monoclonal antibody

CARD11 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68269-1-Ig

产品说明书

CloneNo. 3F5H6

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

BIMP3, CARD11, Carma 1, CARMA1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inMOLT-4 cells, Raji cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inL02 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68269-1-Ig targets CARD11 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 CARD11 fusion protein Ag16468 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 caspase recruitment domain family, member 11
别名 BIMP3, CARD11, Carma 1, CARMA1
计算分子量 1154 aa, 133 kDa
观测分子量 140 kDa
GenBank蛋白编号BC111719
基因名称 CARD11
Gene ID (NCBI) 84433
RRIDAB_3085050
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9BXL7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CARD11 also named as ,BIMP3 or CARMA1, is a 1154 amino acid protein, which contains one PDZ domain, one guanylate kinase-like domain, and one CARD domain. CARD11 localizes in the cytoplasm and co-localized with DPP4 in membrane raft. CARD11 is detected in adult peripheral blood leukocytes, thymus, spleen and liver. CARD11 also is found in CARD11 promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K-562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. CARD11 is involved in the co-stimulatory signal essential for T-cell receptor mediated T-cell activation.

实验方案

Product Specific Protocols
WB protocol for CARD11 antibody 68269-1-IgDownload protocol
IF protocol for CARD11 antibody 68269-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols