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CoraLite® Plus 488-conjugated CARD11 Monoclonal antibody

CARD11 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3F5H6

Cat no : CL488-68269

Print datasheet

Synonyms

Caspase recruitment domain-containing protein 11, CARMA1, Carma 1, CARD-containing MAGUK protein 1, 3F5H6

验证数据展示

  • IF Staining of HepG2 using CL488-68269

    IF Staining of HepG2 using CL488-68269

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 CARD11 antibody (CL488-68269, Clone: 3F5H6 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-68269

    FC experiment of HepG2 using CL488-68269

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated CARD11 Monoclonal antibody (CL488-68269, Clone:3F5H6)(red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (1F8D3) (CL488-66360-1, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 68269-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68269 targets CARD11 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen CARD11 fusion protein Ag16468 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name caspase recruitment domain family, member 11
Synonyms Caspase recruitment domain-containing protein 11, CARMA1, Carma 1, CARD-containing MAGUK protein 1, 3F5H6
Calculated Molecular Weight 1154 aa, 133 kDa
Observed Molecular Weight 140 kDa
GenBank Accession NumberBC111719
Gene Symbol CARD11
Gene ID (NCBI) 84433
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BXL7
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CARD11 also named as ,BIMP3 or CARMA1, is a 1154 amino acid protein, which contains one PDZ domain, one guanylate kinase-like domain, and one CARD domain. CARD11 localizes in the cytoplasm and co-localized with DPP4 in membrane raft. CARD11 is detected in adult peripheral blood leukocytes, thymus, spleen and liver. CARD11 also is found in CARD11 promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K-562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. CARD11 is involved in the co-stimulatory signal essential for T-cell receptor mediated T-cell activation.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CARD11 antibody CL488-68269Download protocol
FC protocol for CL Plus 488 CARD11 antibody CL488-68269Download protocol
Standard Protocols
Click here to view our Standard Protocols