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验证数据展示

  • IF Staining of HepG2 using CL488-68269

    IF Staining of HepG2 using CL488-68269

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 CARD11 antibody (CL488-68269, Clone: 3F5H6 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-68269

    FC experiment of HepG2 using CL488-68269

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated CARD11 Monoclonal antibody (CL488-68269, Clone:3F5H6)(red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (1F8D3) (CL488-66360-1, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated CARD11 Monoclonal antibody

CARD11 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-68269

产品说明书

CloneNo. 3F5H6

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

Caspase recruitment domain-containing protein 11, CARMA1, Carma 1, CARD-containing MAGUK protein 1, 3F5H6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 68269-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68269 targets CARD11 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 CARD11 fusion protein Ag16468 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 caspase recruitment domain family, member 11
别名 Caspase recruitment domain-containing protein 11, CARMA1, Carma 1, CARD-containing MAGUK protein 1, 3F5H6
计算分子量 1154 aa, 133 kDa
观测分子量 140 kDa
GenBank蛋白编号BC111719
基因名称 CARD11
Gene ID (NCBI) 84433
RRIDAB_3672984
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9BXL7
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CARD11 also named as ,BIMP3 or CARMA1, is a 1154 amino acid protein, which contains one PDZ domain, one guanylate kinase-like domain, and one CARD domain. CARD11 localizes in the cytoplasm and co-localized with DPP4 in membrane raft. CARD11 is detected in adult peripheral blood leukocytes, thymus, spleen and liver. CARD11 also is found in CARD11 promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K-562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. CARD11 is involved in the co-stimulatory signal essential for T-cell receptor mediated T-cell activation.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CARD11 antibody CL488-68269Download protocol
FC protocol for CL Plus 488 CARD11 antibody CL488-68269Download protocol
Standard Protocols
Click here to view our Standard Protocols