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验证数据展示

  • WB analysis using 68657-1-Ig

    WB analysis using 68657-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68657-1-Ig (CARD9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of THP-1 using 68657-1-Ig

    WB analysis of THP-1 using 68657-1-Ig

    WB result of CARD9 antibody (68657-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CARD9 transfected THP-1 cells.

  • IF Staining of HeLa using 68657-1-Ig

    IF Staining of HeLa using 68657-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CARD9 antibody (68657-1-Ig, Clone: 2F3E10 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

  • Featured Product
  • KD/KO Validated

CARD9 Monoclonal antibody

CARD9 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68657-1-Ig

Print datasheet

CloneNo. 2F3E10

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

hCARD9, Caspase recruitment domain-containing protein 9, 2F3E10

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inNK-92 cells, HL-60 cells, TF-1 cells, THP-1 cells, U-937 cells, MOLT-4 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68657-1-Ig targets CARD9 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen CARD9 fusion protein Ag33643 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name caspase recruitment domain family, member 9
Synonyms hCARD9, Caspase recruitment domain-containing protein 9, 2F3E10
Calculated Molecular Weight 62 kDa
Observed Molecular Weight55-65 kDa
GenBank Accession NumberBC008877
Gene Symbol CARD9
Gene ID (NCBI) 64170
RRIDAB_3670399
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9H257
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CARD9, a member of the caspase recruitment domain (CARD) protein family, plays an important regulatory role in cell apoptosis. Defects in CARD9 are the cause of familial candidiasis type 2 (CANDF2) characterized by impaired clearance of fungal infections and results in colonization and infections of the mucosa or skin, predominantly with Candida albicans. CARD9 is highly expressed in the spleen and regulates activation of NF-kappa-B by interaction with postive regulator of apoptosis BCL10 via the CARD domain.

实验方案

Product Specific Protocols
WB protocol for CARD9 antibody 68657-1-IgDownload protocol
IF protocol for CARD9 antibody 68657-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols