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验证数据展示

  • WB analysis using 68657-1-Ig (same clone as 68657-1-PBS)

    WB analysis using 68657-1-Ig (same clone as 68657-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68657-1-Ig (CARD9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68657-1-PBS in a different storage buffer formulation.

  • WB analysis of THP-1 using 68657-1-Ig (same clone as 68657-1-PBS)

    WB analysis of THP-1 using 68657-1-Ig (same clone as 68657-1-PBS)

    WB result of CARD9 antibody (68657-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CARD9 transfected THP-1 cells. This data was developed using the same antibody clone with 68657-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68657-1-Ig (same clone as 68657-1-PBS)

    IF Staining of HeLa using 68657-1-Ig (same clone as 68657-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CARD9 antibody (68657-1-Ig, Clone: 2F3E10 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 68657-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

CARD9 Monoclonal antibody, PBS Only

CARD9 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68657-1-PBS

产品说明书

CloneNo. 2F3E10

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

hCARD9, Caspase recruitment domain-containing protein 9, 2F3E10

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68657-1-PBS targets CARD9 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 CARD9 fusion protein Ag33643 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 caspase recruitment domain family, member 9
别名 hCARD9, Caspase recruitment domain-containing protein 9, 2F3E10
计算分子量 62 kDa
观测分子量55-65 kDa
GenBank蛋白编号BC008877
基因名称 CARD9
Gene ID (NCBI) 64170
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9H257
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CARD9, a member of the caspase recruitment domain (CARD) protein family, plays an important regulatory role in cell apoptosis. Defects in CARD9 are the cause of familial candidiasis type 2 (CANDF2) characterized by impaired clearance of fungal infections and results in colonization and infections of the mucosa or skin, predominantly with Candida albicans. CARD9 is highly expressed in the spleen and regulates activation of NF-kappa-B by interaction with postive regulator of apoptosis BCL10 via the CARD domain.