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CARD9 Monoclonal antibody, PBS Only

CARD9 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F3E10

Cat no : 68657-1-PBS

Print datasheet

Synonyms

hCARD9, Caspase recruitment domain-containing protein 9, 2F3E10

验证数据展示

  • WB analysis using 68657-1-Ig (same clone as 68657-1-PBS)

    WB analysis using 68657-1-Ig (same clone as 68657-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68657-1-Ig (CARD9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68657-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68657-1-Ig (same clone as 68657-1-PBS)

    IF Staining of HeLa using 68657-1-Ig (same clone as 68657-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CARD9 antibody (68657-1-Ig, Clone: 2F3E10 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 68657-1-PBS in a different storage buffer formulation.

产品信息

68657-1-PBS targets CARD9 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen CARD9 fusion protein Ag33643 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name caspase recruitment domain family, member 9
Synonyms hCARD9, Caspase recruitment domain-containing protein 9, 2F3E10
Calculated Molecular Weight 62 kDa
Observed Molecular Weight55-65 kDa
GenBank Accession NumberBC008877
Gene Symbol CARD9
Gene ID (NCBI) 64170
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9H257
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CARD9, a member of the caspase recruitment domain (CARD) protein family, plays an important regulatory role in cell apoptosis. Defects in CARD9 are the cause of familial candidiasis type 2 (CANDF2) characterized by impaired clearance of fungal infections and results in colonization and infections of the mucosa or skin, predominantly with Candida albicans. CARD9 is highly expressed in the spleen and regulates activation of NF-kappa-B by interaction with postive regulator of apoptosis BCL10 via the CARD domain.