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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67885-1-Ig (same clone as 67885-1-PBS)

    WB analysis using 67885-1-Ig (same clone as 67885-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67885-1-Ig (CBFB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67885-1-PBS in a different storage buffer formulation.

  • FC experiment of K-562 using 67885-1-Ig (same clone as 67885-1-PBS)

    FC experiment of K-562 using 67885-1-Ig (same clone as 67885-1-PBS)

    1X10^6 K-562 cells were intracellularly stained with 0.5 ug Anti-Human CBFB (67885-1-Ig, Clone:1D7F2) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67885-1-PBS in a different storage buffer formulation.

CBFB Monoclonal antibody, PBS Only

CBFB Monoclonal Antibody for Indirect ELISA, WB
Cat No. 67885-1-PBS

Print datasheet

CloneNo. 1D7F2

Host / Isotype

Mouse / IgG1

Reactivity

rat, human

Applications

Indirect ELISA, WB

Synonyms

PEBP2 beta, PEA2-beta, Core-binding factor subunit beta, CBF-beta, CBF beta

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

67885-1-PBS targets CBFB in Indirect ELISA, WB applications and shows reactivity with rat, human samples.

Tested Applications Indirect ELISA, WB Application Description
Tested Reactivity rat, human
Immunogen CBFB fusion protein Ag31183 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name core-binding factor, beta subunit
Synonyms PEBP2 beta, PEA2-beta, Core-binding factor subunit beta, CBF-beta, CBF beta
Calculated Molecular Weight 182 aa, 22 kDa
GenBank Accession NumberBC018509
Gene Symbol CBFB
Gene ID (NCBI) 865
RRIDAB_2918642
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13951
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CBFB (core binding factor subunit β), forms the heterodimeric complex core-binding factor (CBF). As a critical transcription factor, CBFB is frequently mutated in breast cancer and considered to be of significance in the pathogenesis of cancer.(PMID: 29932212) The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters.) It has calculated molecular weight around 22kDa.