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验证数据展示

  • WB analysis using 67885-1-Ig

    WB analysis using 67885-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67885-1-Ig (CBFB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • FC experiment of K-562 using 67885-1-Ig

    FC experiment of K-562 using 67885-1-Ig

    1X10^6 K-562 cells were intracellularly stained with 0.5 ug Anti-Human CBFB (67885-1-Ig, Clone:1D7F2) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CBFB Monoclonal antibody

CBFB Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 67885-1-Ig

产品说明书

CloneNo. 1D7F2

宿主/亚型

Mouse / IgG1

种属反应性

human, rat

应用

WB, FC (Intra), ELISA

别名

PEBP2 beta, PEA2-beta, Core-binding factor subunit beta, CBF-beta, CBF beta

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, HEK-293 cells, MOLT-4 cells, K-562 cells, HSC-T6 cells
Positive FC (Intra) detected inK-562 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67885-1-Ig targets CBFB in WB, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, rat
免疫原 CBFB fusion protein Ag31183 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 core-binding factor, beta subunit
别名 PEBP2 beta, PEA2-beta, Core-binding factor subunit beta, CBF-beta, CBF beta
计算分子量 182 aa, 22 kDa
GenBank蛋白编号BC018509
基因名称 CBFB
Gene ID (NCBI) 865
RRIDAB_2918642
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ13951
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CBFB (core binding factor subunit β), forms the heterodimeric complex core-binding factor (CBF). As a critical transcription factor, CBFB is frequently mutated in breast cancer and considered to be of significance in the pathogenesis of cancer.(PMID: 29932212) The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters.) It has calculated molecular weight around 22kDa.

实验方案

Product Specific Protocols
WB protocol for CBFB antibody 67885-1-IgDownload protocol
FC protocol for CBFB antibody 67885-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols