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验证数据展示

  • WB analysis using 67885-1-Ig

    WB analysis using 67885-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67885-1-Ig (CBFB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • FC experiment of K-562 using 67885-1-Ig

    FC experiment of K-562 using 67885-1-Ig

    1X10^6 K-562 cells were intracellularly stained with 0.5 ug Anti-Human CBFB (67885-1-Ig, Clone:1D7F2) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CBFB Monoclonal antibody

CBFB Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 67885-1-Ig

Print datasheet

CloneNo. 1D7F2

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, FC (Intra), ELISA

Synonyms

PEBP2 beta, PEA2-beta, Core-binding factor subunit beta, CBF-beta, CBF beta

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, HEK-293 cells, MOLT-4 cells, K-562 cells, HSC-T6 cells
Positive FC (Intra) detected inK-562 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67885-1-Ig targets CBFB in WB, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, rat
Immunogen CBFB fusion protein Ag31183 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name core-binding factor, beta subunit
Synonyms PEBP2 beta, PEA2-beta, Core-binding factor subunit beta, CBF-beta, CBF beta
Calculated Molecular Weight 182 aa, 22 kDa
GenBank Accession NumberBC018509
Gene Symbol CBFB
Gene ID (NCBI) 865
RRIDAB_2918642
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13951
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CBFB (core binding factor subunit β), forms the heterodimeric complex core-binding factor (CBF). As a critical transcription factor, CBFB is frequently mutated in breast cancer and considered to be of significance in the pathogenesis of cancer.(PMID: 29932212) The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters.) It has calculated molecular weight around 22kDa.

实验方案

Product Specific Protocols
WB protocol for CBFB antibody 67885-1-IgDownload protocol
FC protocol for CBFB antibody 67885-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols