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验证数据展示

  • FC experiment of K-562 using CL488-67885

    FC experiment of K-562 using CL488-67885

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CBFB (CL488-67885, Clone:1D7F2) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated CBFB Monoclonal antibody

CBFB Monoclonal Antibody for FC (Intra)
Cat No. CL488-67885

产品说明书

CloneNo. 1D7F2

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

FC (Intra)

别名

PEBP2 beta, PEA2-beta, Core-binding factor subunit beta, CBF-beta, CBF beta

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inK-562 cells
For other applications, we recommend the unconjugated version of this antibody, 67885-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67885 targets CBFB in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 CBFB fusion protein Ag31183 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 core-binding factor, beta subunit
别名 PEBP2 beta, PEA2-beta, Core-binding factor subunit beta, CBF-beta, CBF beta
计算分子量 182 aa, 22 kDa
GenBank蛋白编号BC018509
基因名称 CBFB
Gene ID (NCBI) 865
RRIDAB_2923816
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ13951
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CBFB (core binding factor subunit β), forms the heterodimeric complex core-binding factor (CBF). As a critical transcription factor, CBFB is frequently mutated in breast cancer and considered to be of significance in the pathogenesis of cancer.(PMID: 29932212) The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters.) It has calculated molecular weight around 22kDa.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 CBFB antibody CL488-67885Download protocol
Standard Protocols
Click here to view our Standard Protocols