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验证数据展示

  • FC experiment of K-562 using CL488-67885

    FC experiment of K-562 using CL488-67885

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CBFB (CL488-67885, Clone:1D7F2) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated CBFB Monoclonal antibody

CBFB Monoclonal Antibody for FC (Intra)
Cat No. CL488-67885

Print datasheet

CloneNo. 1D7F2

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC (Intra)

Synonyms

PEBP2 beta, PEA2-beta, Core-binding factor subunit beta, CBF-beta, CBF beta

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488

Size: 
100 μL

¥2800/ 100 μL

浓度: 1000 μg/ml

库存：现货

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经过测试的应用

Positive FC (Intra) detected inK-562 cells
For other applications, we recommend the unconjugated version of this antibody, 67885-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67885 targets CBFB in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen CBFB fusion protein Ag31183 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name core-binding factor, beta subunit
Synonyms PEBP2 beta, PEA2-beta, Core-binding factor subunit beta, CBF-beta, CBF beta
Calculated Molecular Weight 182 aa, 22 kDa
GenBank Accession NumberBC018509
Gene Symbol CBFB
Gene ID (NCBI) 865
RRIDAB_2923816
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13951
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CBFB (core binding factor subunit β), forms the heterodimeric complex core-binding factor (CBF). As a critical transcription factor, CBFB is frequently mutated in breast cancer and considered to be of significance in the pathogenesis of cancer.(PMID: 29932212) The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters.) It has calculated molecular weight around 22kDa.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 CBFB antibody CL488-67885Download protocol
Standard Protocols
Click here to view our Standard Protocols