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CBLB Monoclonal antibody, PBS Only

CBLB Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

human, mouse, pig, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2D3A2

Cat no : 66353-1-PBS

Print datasheet

Synonyms

CBLB, Nbla00127, RING finger protein 56, RNF56, SH3 binding protein CBL B

验证数据展示

  • WB analysis of RAW 264.7 using 66353-1-Ig (same clone as 66353-1-PBS)

    WB analysis of RAW 264.7 using 66353-1-Ig (same clone as 66353-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66353-1-Ig (CBLB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66353-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 66353-1-Ig (same clone as 66353-1-PBS)

    IHC staining of human heart using 66353-1-Ig (same clone as 66353-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66353-1-Ig (CBLB Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66353-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 66353-1-Ig (same clone as 66353-1-PBS)

    IHC staining of human heart using 66353-1-Ig (same clone as 66353-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66353-1-Ig (CBLB Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66353-1-PBS in a different storage buffer formulation.

  • IF Staining of RAW 264.7 using 66353-1-Ig (same clone as 66353-1-PBS)

    IF Staining of RAW 264.7 using 66353-1-Ig (same clone as 66353-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed RAW 264.7 cells using 66353-1-Ig(CBLB antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66353-1-PBS in a different storage buffer formulation.

产品信息

66353-1-PBS targets CBLB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, pig, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, pig, rat
Immunogen CBLB fusion protein Ag18757 种属同源性预测
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Full Name Cas-Br-M (murine) ecotropic retroviral transforming sequence b
Synonyms CBLB, Nbla00127, RING finger protein 56, RNF56, SH3 binding protein CBL B
Calculated Molecular Weight 982 aa, 109 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC032851
Gene Symbol CBLB
Gene ID (NCBI) 868
RRIDAB_2881733
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13191
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CBLB(E3 ubiquitin-protein ligase CBL-B) is also known as RNF56 and plays a necessary role to prevent the inappropriate activation of NF-B in response to lipopolysaccharide and to polymicrobial sepsis. CBLB can be phosphorylated on tyrosine and serine residues upon TCR or BCR activation, and upon various types of cell stimulation.. It also has a crucial role in the prevention of chronic inflammation and autoimmunity. This protein has 3 isoforms produced by alternative splicing.