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CBLB Monoclonal antibody

CBLB Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

human, mouse, pig, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

2D3A2

Cat no : 66353-1-Ig

Print datasheet

Synonyms

CBLB, Nbla00127, RING finger protein 56, RNF56, SH3 binding protein CBL B

验证数据展示

  • WB analysis of RAW 264.7 using 66353-1-Ig

    WB analysis of RAW 264.7 using 66353-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66353-1-Ig (CBLB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 66353-1-Ig

    IHC staining of human heart using 66353-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66353-1-Ig (CBLB Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human heart using 66353-1-Ig

    IHC staining of human heart using 66353-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66353-1-Ig (CBLB Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of RAW 264.7 using 66353-1-Ig

    IF Staining of RAW 264.7 using 66353-1-Ig

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed RAW 264.7 cells using 66353-1-Ig(CBLB antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inRAW 264.7 cells, rat heart tissue
Positive IHC detected inhuman heart tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRAW 264.7 cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66353-1-Ig targets CBLB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, pig, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse, pig, rat
Cited Reactivityhuman, mouse
Immunogen CBLB fusion protein Ag18757 种属同源性预测
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Full Name Cas-Br-M (murine) ecotropic retroviral transforming sequence b
Synonyms CBLB, Nbla00127, RING finger protein 56, RNF56, SH3 binding protein CBL B
Calculated Molecular Weight 982 aa, 109 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC032851
Gene Symbol CBLB
Gene ID (NCBI) 868
RRIDAB_2881733
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13191
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CBLB(E3 ubiquitin-protein ligase CBL-B) is also known as RNF56 and plays a necessary role to prevent the inappropriate activation of NF-B in response to lipopolysaccharide and to polymicrobial sepsis. CBLB can be phosphorylated on tyrosine and serine residues upon TCR or BCR activation, and upon various types of cell stimulation.. It also has a crucial role in the prevention of chronic inflammation and autoimmunity. This protein has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for CBLB antibody 66353-1-IgDownload protocol
IHC protocol for CBLB antibody 66353-1-IgDownload protocol
IF protocol for CBLB antibody 66353-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC,IF

J Exp Clin Cancer Res

GABA induced by sleep deprivation promotes the proliferation and migration of colon tumors through miR-223-3p endogenous pathway and exosome pathway

Authors - Haijun Bao
View Article