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验证数据展示

  • IF Staining of RAW 264.7 using CL594-66353

    IF Staining of RAW 264.7 using CL594-66353

    Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using CoraLite®594 CBLB antibody (CL594-66353, Clone: 2D3A2 ) at dilution of 1:100. DAPI (blue).

CoraLite®594-conjugated CBLB Monoclonal antibody

CBLB Monoclonal Antibody for IF/ICC
Cat No. CL594-66353

产品说明书

CloneNo. 2D3A2

宿主/亚型

Mouse / IgG3

种属反应性

human, mouse, pig, rat

应用

IF/ICC

别名

CBLB, DKFZp686J10223, DKFZp779A0729, DKFZp779F1443, FLJ36865, FLJ41152, Nbla00127, RING finger protein 56, RNF56, SH3 binding protein CBL B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inRAW 264.7 cells, U2OS cells
For other applications, we recommend the unconjugated version of this antibody, 66353-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66353 targets CBLB in IF/ICC applications and shows reactivity with human, mouse, pig, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, pig, rat
免疫原 CBLB fusion protein Ag18757 种属同源性预测
宿主/亚型 Mouse / IgG3
抗体类别 Monoclonal
产品类型 Antibody
全称 Cas-Br-M (murine) ecotropic retroviral transforming sequence b
别名 CBLB, DKFZp686J10223, DKFZp779A0729, DKFZp779F1443, FLJ36865, FLJ41152, Nbla00127, RING finger protein 56, RNF56, SH3 binding protein CBL B
计算分子量 982 aa, 109 kDa
观测分子量 110 kDa
GenBank蛋白编号BC032851
基因名称 CBLB
Gene ID (NCBI) 868
RRIDAB_2919978
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13191
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CBLB(E3 ubiquitin-protein ligase CBL-B) is also known as RNF56 and plays a necessary role to prevent the inappropriate activation of NF-B in response to lipopolysaccharide and to polymicrobial sepsis. CBLB can be phosphorylated on tyrosine and serine residues upon TCR or BCR activation, and upon various types of cell stimulation.. It also has a crucial role in the prevention of chronic inflammation and autoimmunity. This protein has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
IF protocol for CL594 CBLB antibody CL594-66353Download protocol
Standard Protocols
Click here to view our Standard Protocols