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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated EIF4E Polyclonal antibody

EIF4E Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11149

Print datasheet

Synonyms

mRNA cap-binding protein, eIF-4F 25 kDa subunit, EIF4F, EIF4EL1, eIF-4E

验证数据展示

  • IF Staining of HepG2 using CL488-11149

    IF Staining of HepG2 using CL488-11149

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 EIF4E antibody (CL488-11149) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-11149

    FC experiment of HepG2 using CL488-11149

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human EIF4E (CL488-11149) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy EIF4E Ready-To-Use IHC Kit. EIF4E primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11149-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11149 targets EIF4E in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen EIF4E fusion protein Ag1626 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name eukaryotic translation initiation factor 4E
Synonyms mRNA cap-binding protein, eIF-4F 25 kDa subunit, EIF4F, EIF4EL1, eIF-4E
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 26-29 kDa, 50 kDa
GenBank Accession NumberBC012611
Gene Symbol EIF4E
Gene ID (NCBI) 1977
RRIDAB_2919014
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP06730
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Eukaryotic translation initiation factor 4E, also known as eIF4E, is a protein that in humans is encoded by the EIF4E gene. eIF4E is the mRNA cap-binding protein, known as a general initiation factor allowing for mRNA-ribosome interaction and cap-dependent translation in eukaryotic cells. eIF4E is a polypeptide that exists as both a free form and as part of the eIF4F pre-initiation complex. Regulation of eIF4E may be achieved via three distinct mechanisms: transcription, phosphorylation, and inhibitory proteins.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 EIF4E antibody CL488-11149Download protocol
FC protocol for CL Plus 488 EIF4E antibody CL488-11149Download protocol
Standard Protocols
Click here to view our Standard Protocols