验证数据展示
经过测试的应用
| Positive WB detected in | human heart tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy NCBP2 Ready-To-Use IHC Kit. NCBP2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11950-1-AP targets CBP20 in WB, IHC, IF/ICC, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, RIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | CBP20 fusion protein Ag2550 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nuclear cap binding protein subunit 2, 20kDa |
| 别名 | NCBP2, NCBP-interacting protein 1, NCBP 20 kDa subunit, Cell proliferation-inducing gene 55 protein, CBC2 |
| 计算分子量 | 156 aa, 18 kDa |
| 观测分子量 | 18 kDa |
| GenBank蛋白编号 | BC001255 |
| 基因名称 | NCBP2 |
| Gene ID (NCBI) | 22916 |
| RRID | AB_2150142 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P52298 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CBP20 antibody 11950-1-AP | Download protocol |
| IHC protocol for CBP20 antibody 11950-1-AP | Download protocol |
| IF protocol for CBP20 antibody 11950-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
iScience Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells. | ||
Eur J Med Res N7-methylguanosin regulators-mediated methylation modification patterns and characterization of the immune microenvironment in lower-grade glioma | ||
Heliyon CircZMYM2 plays a pivotal role in osteosarcoma by regulating the translation of NACA and ARPC1B | ||