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CBP20 Polyclonal antibody

CBP20 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11950-1-AP

Print datasheet

Synonyms

NCBP2, NCBP-interacting protein 1, NCBP 20 kDa subunit, Cell proliferation-inducing gene 55 protein, CBC2

验证数据展示

  • WB analysis of human heart using 11950-1-AP

    WB analysis of human heart using 11950-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 11950-1-AP (CBP20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 11950-1-AP

    IHC staining of mouse brain using 11950-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11950-1-AP (CBP20 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NCBP2 antibody included
  • IHC staining of mouse brain using 11950-1-AP

    IHC staining of mouse brain using 11950-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11950-1-AP (CBP20 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NCBP2 antibody included
  • IF Staining of HeLa using 11950-1-AP

    IF Staining of HeLa using 11950-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CBP20 antibody (11950-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inhuman heart tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NCBP2 Ready-To-Use IHC Kit. NCBP2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11950-1-AP targets CBP20 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen CBP20 fusion protein Ag2550 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nuclear cap binding protein subunit 2, 20kDa
Synonyms NCBP2, NCBP-interacting protein 1, NCBP 20 kDa subunit, Cell proliferation-inducing gene 55 protein, CBC2
Calculated Molecular Weight 156 aa, 18 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC001255
Gene Symbol NCBP2
Gene ID (NCBI) 22916
RRIDAB_2150142
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP52298
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CBP20 antibody 11950-1-APDownload protocol
IHC protocol for CBP20 antibody 11950-1-APDownload protocol
IF protocol for CBP20 antibody 11950-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

iScience

Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.

Authors - Mio Iwasaki
View Article
humanWB,IHC

Eur J Med Res

N7-methylguanosin regulators-mediated methylation modification patterns and characterization of the immune microenvironment in lower-grade glioma

Authors - Aierpati Maimaiti
View Article