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验证数据展示

  • WB analysis using 83736-4-RR

    WB analysis using 83736-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83736-4-RR (CBR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 83736-4-RR

    IF Staining of HeLa using 83736-4-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CBR1 antibody (83736-4-RR, Clone: 240707G1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83736-4-RR

    Affinity and Kinetic Characterization of 83736-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83736-4-RR against Human CBR1 were performed. The affinity constant is 13.7 nM.

CBR1 Recombinant antibody

CBR1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83736-4-RR

产品说明书

CloneNo. 240707G1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

EC:1.1.1.184, carbonyl reductase 1, Alcohol dehydrogenase [NAD(P)+] CBR1, 240707G1, 20-beta-hydroxysteroid dehydrogenase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, A549 cells, HEK-293T cells, MCF-7 cells, SKOV-3 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83736-4-RR targets CBR1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 CBR1 fusion protein Ag34830 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 carbonyl reductase 1
别名 EC:1.1.1.184, carbonyl reductase 1, Alcohol dehydrogenase [NAD(P)+] CBR1, 240707G1, 20-beta-hydroxysteroid dehydrogenase
计算分子量 30 kDa
观测分子量 30 kDa
GenBank蛋白编号BC002511
基因名称 CBR1
Gene ID (NCBI) 873
RRIDAB_3671336
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP16152
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CBR1 belongs to the short-chain dehydrogenases/reductases (SDR) family, which function as NADPH-dependent oxidoreductases having wide specificity for carbonyl compounds, such as quinones, prostaglandins, and various xenobiotics.

实验方案

Product Specific Protocols
WB protocol for CBR1 antibody 83736-4-RRDownload protocol
IF protocol for CBR1 antibody 83736-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols