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验证数据展示

  • IF Staining of HeLa using CL488-67861

    IF Staining of HeLa using CL488-67861

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 CBS antibody (CL488-67861, Clone: 1E4F10 ) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HeLa using CL488-67861

    FC experiment of HeLa using CL488-67861

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CBS (CL488-67861, Clone:1E4F10) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated CBS Monoclonal antibody

CBS Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67861

产品说明书

CloneNo. 1E4F10

宿主/亚型

Mouse / IgG2b

种属反应性

human, rat

应用

IF/ICC, FC (Intra)

别名

EC:4.2.1.22, Cystathionine beta-synthase, cystathionine beta synthase, Beta-thionase, Beta thionase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67861-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67861 targets CBS in IF/ICC, FC (Intra) applications and shows reactivity with human, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, rat
免疫原 CBS fusion protein Ag7008 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 cystathionine-beta-synthase
别名 EC:4.2.1.22, Cystathionine beta-synthase, cystathionine beta synthase, Beta-thionase, Beta thionase
计算分子量 61 kDa
观测分子量 61-63 kDa
GenBank蛋白编号BC000440
基因名称 CBS
Gene ID (NCBI) 875
RRIDAB_2934577
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP35520
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The CBS gene encodes cystathionine beta-synthase, which catalyzes the first irreversible step of transsulfuration. The CBS enzyme is a homotetramer of 63-kD subunits and requires pyridoxal phosphate and heme for activity(PMID:11230183). CBS protein is localized in most areas of the brain, but predominantly in the cell bodies and neuronal processes of Purkinje cells and Ammon's horn neurons(PMID:12588964).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CBS antibody CL488-67861Download protocol
FC protocol for CL Plus 488 CBS antibody CL488-67861Download protocol
Standard Protocols
Click here to view our Standard Protocols